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Series GSE64238 Query DataSets for GSE64238
Status Public on Sep 21, 2020
Title Metabolic adaptation of white adipose tissue to acute, short-term environmental oxygen restriction in mice
Organism Mus musculus
Experiment type Expression profiling by array
Summary White adipose tissue (WAT) expansion during e.g. obesity reduces oxygen availability in WAT in mice. Little is known on the adaptation of WAT to mild environmental oxygen restriction (OxR). Therefore, we studied metabolic adaptation to acute OxR in fasted, diet-induced moderately obese mice that were exposed to mild hypoxic (12% O2) or normoxic (20.9% O2) conditions for only 6 hours. Adaptation was assessed by determination of amino acids and (acyl)carnitines levels in serum and WAT, and by whole genome expression analysis in WAT. Adaptation was also assessed during the exposure using indirect calorimetry. We found that OxR reduced mitochondrial oxidation at whole-body level, as shown by a reduction in whole-body oxygen consumption and an increase in serum long-chain acylcarnitine levels. WAT did not seem to contribute to this serum profile since only short-chain acylcarnitines were increased in WAT and gene expression analysis indicated an increase in mitochondrial oxidation, based on coordinate down-regulation of Sirt4, Gpam and Chchd3/Minos3. In addition, OxR did not induce oxidative stress in obese WAT, but increased molecular pathways involved in cell growth and proliferation. OxR increased levels of tyrosine, lysine and ornithine in serum and of leucine/isoleucine in WAT. This study shows that OxR limits oxidative phosphorylation at whole-body level, but in WAT compensatory mechanisms seem to operate. The down-regulation of the mitochondria-related genes Sirt4, Gpam, and Chchd3 may be considered as a biomarker profile for WAT mitochondrial reprogramming in response to acute exposure to limited oxygen availability.
 
Overall design Two-condition experiment, normoxia (20.9% oxygen) vs. hypoxia (12% oxygen) treated mice. Biological replicates: 12 control (normoxia) replicates, 10 hypoxia replicates.
 
Contributor(s) Duivenvoorde LP, van Schothorst EM, van der Stelt I, Hoek-van den Hil E, Keijer J
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Submission date Dec 16, 2014
Last update date Sep 22, 2020
Contact name Evert M. van Schothorst
E-mail(s) evert.vanschothorst@wur.nl
Organization name Wageningen University
Lab Human and Animal Physiology
Street address De Elst 1
City Wageningen
ZIP/Postal code 6708 WD
Country Netherlands
 
Platforms (1)
GPL13912 Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Feature Number version)
Samples (22)
GSM1566813 WAT normoxia replicate 01
GSM1566814 WAT normoxia replicate 02
GSM1566815 WAT normoxia replicate 03
Relations
BioProject PRJNA270508

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE64238_RAW.tar 466.9 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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