|
Status |
Public on Jul 25, 2020 |
Title |
Comparison of expression profiles for Nicotiana benthamiana agroinfiltrated with caryophyllene synthase and SNARE-RNAi |
Organism |
Nicotiana benthamiana |
Experiment type |
Expression profiling by high throughput sequencing
|
Summary |
Here we show for the first time that vesicle fusion is one of multiple pathways in plant cells involved in terpene emissions. We demonstrate a positive feedback on terpene production when vesicle fusion is inhibited in cells producing high levels of terpenes, which relates to an induced proteasome malfunction.
|
|
|
Overall design |
N. benthamiana leaves were infiltrated with Agrobacterium containing the following expression constructs: (1) pBin+DsRed-RNAi, (2) MtVAMP721e-RNAi+DsRed-RNAi, (3) CST+DsRed-RNAi, (4) CST+MtVAMP721e-RNAi (six plants per construct, one leaf per plant). Infiltrated leaves were harvested seven days post agroinfiltration. Per sample two leaves were pooled, resulting in three biological replicates for each treatment. Followed by deep sequencing, using Illumina Hiseq 2000.
|
|
|
Contributor(s) |
van der Krol A, Ting H |
Citation missing |
Has this study been published? Please login to update or notify GEO. |
Submission date |
Aug 04, 2014 |
Last update date |
Jul 26, 2020 |
Contact name |
Hieng-Ming Ting |
E-mail(s) |
jimmytinghm@gmail.com
|
Organization name |
Wageningen University
|
Department |
Laboratory of Plant Physiology
|
Street address |
Droevendaalsesteeg 1
|
City |
Wageningen |
State/province |
Gelderland |
ZIP/Postal code |
6708 PB |
Country |
Netherlands |
|
|
Platforms (1) |
GPL19037 |
Illumina HiSeq 2000 (Nicotiana benthamiana) |
|
Samples (12)
|
|
Relations |
BioProject |
PRJNA257401 |
SRA |
SRP045229 |