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Series GSE60061 Query DataSets for GSE60061
Status Public on Jul 25, 2020
Title Comparison of expression profiles for Nicotiana benthamiana agroinfiltrated with caryophyllene synthase and SNARE-RNAi
Organism Nicotiana benthamiana
Experiment type Expression profiling by high throughput sequencing
Summary Here we show for the first time that vesicle fusion is one of multiple pathways in plant cells involved in terpene emissions. We demonstrate a positive feedback on terpene production when vesicle fusion is inhibited in cells producing high levels of terpenes, which relates to an induced proteasome malfunction.
 
Overall design N. benthamiana leaves were infiltrated with Agrobacterium containing the following expression constructs: (1) pBin+DsRed-RNAi, (2) MtVAMP721e-RNAi+DsRed-RNAi, (3) CST+DsRed-RNAi, (4) CST+MtVAMP721e-RNAi (six plants per construct, one leaf per plant). Infiltrated leaves were harvested seven days post agroinfiltration. Per sample two leaves were pooled, resulting in three biological replicates for each treatment. Followed by deep sequencing, using Illumina Hiseq 2000.
 
Contributor(s) van der Krol A, Ting H
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Submission date Aug 04, 2014
Last update date Jul 26, 2020
Contact name Hieng-Ming Ting
E-mail(s) jimmytinghm@gmail.com
Organization name Wageningen University
Department Laboratory of Plant Physiology
Street address Droevendaalsesteeg 1
City Wageningen
State/province Gelderland
ZIP/Postal code 6708 PB
Country Netherlands
 
Platforms (1)
GPL19037 Illumina HiSeq 2000 (Nicotiana benthamiana)
Samples (12)
GSM1464452 CST-DsRed_1
GSM1464453 CST-DsRed_2
GSM1464454 CST-DsRed_3
Relations
BioProject PRJNA257401
SRA SRP045229

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE60061_RAW.tar 41.7 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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