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| Status |
Public on Jul 13, 2020 |
| Title |
Different lipid forms of omega-3 and their effect on small intestine in mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Omega - 3 fatty acids of marine origin exert beneficial effects on lipid metabolism and can protect against insulin resistance in high fat diet (HFD)-fed animals. Simultaneously, recent studies showed that different lipid forms could have numerous consequences regarding the regulation of energy balance, nutrient absorption, and substrate metabolism. Indeed, when omega-3 was provided as triglycerides (TG, i.e. fish oil), it induced dose-dependently the expression of genes involved in lipid metabolism as well as fatty acid oxidation in small intestine of C57BL/6 mice fed various HFDs. As the underlying mechanism(s) explaining the differences in EPA/DHA bioavailability among various lipid forms of Omega-3 is not entirely clear, we performed a mouse study (n=8 per group) using purified HFDs with control HFD based on corn oil (cHF) and part of the lipids were replaced by omega-3 fish lipids in different forms: as either TG (cHF-F), marine phospholipids (PL; Krill oil, given at two different doses Krill-low (Krill-L) and Krill-high (Krill-H)), and as wax esters in the extract from the zooplankton Calanus finmarchicus (Calanus oil CAL-L representing same omega-3 levels as Krill-L diet). As a healthy control we fed a subset of mice standard chow (STD). All mice were fed their diet for 8 weeks and after sacrifice, whole small intestine was isolated, frozen and used for RNA isolation and microarray gene expression analysis using 8x60K Agilent arrays. Results showed that PL-H versus control cHFc induced specifically metabolic lipid pathways, while TG and PL-L mainly affected cytoskeleton regulation.
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| Overall design |
Adult male C57BL/6N mice were initially fed cHF diet (except STD group) for one week and then fed experimetal diets (cHF, cHF-F, Krill - H, Krill-L and CAL-L) for 8 weeks. After sacrifice, whole small intestine was rapidly removed,opened on cold pad ,gently wiped of the remains of lumenal content, weighed, and frozen in liquid nitrogen and used for RNA isolation and whole genome gene expression microarray hybridisation using Agilent 8x60K arrays.
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| Contributor(s) |
van Schothorst EM, Kucharikova P, Bunschoten A, Horakova O, Rossmeisl M, Kopecky J, Keijer J |
| Citation |
https://doi.org/10.3390/nu12072037
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| Submission date |
Jan 04, 2017 |
| Last update date |
Jul 13, 2020 |
| Contact name |
Evert M. van Schothorst |
| E-mail(s) |
evert.vanschothorst@wur.nl
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| Organization name |
Wageningen University
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| Lab |
Human and Animal Physiology
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| Street address |
De Elst 1
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| City |
Wageningen |
| ZIP/Postal code |
6708 WD |
| Country |
Netherlands |
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| Platforms (1) |
| GPL13912 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Feature Number version) |
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| Samples (48)
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| Relations |
| BioProject |
PRJNA360138 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE93151_RAW.tar |
293.6 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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