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| Status |
Public on Jan 08, 2020 |
| Title |
Peripheral blood mononuclear cells as a source of cold exposure biomarkers |
| Organism |
Mustela putorius furo |
| Experiment type |
Expression profiling by array
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| Summary |
Male ferrets, aged 3 months, were divided into two group: one group remained at 22 degrees Celsius, while the other group was acclimatized to 4 degrees Celsius for one week. After sacrification, inguinal and periaortic white adipose tissues were dissected, and peripheral blood mononuclear cells (PBMC) were isolated. The three tissues are used for RNA isolation and subsequent global gene expression profiling using custom Agilent ferret-specific 2x400K microarrays. Data analysis indicated that the cold exposure induce a clear gene expression response of some genes in the inguinal and periaortic white adipose tissue as in PBMC. These genes could be defined as a biomakers of the effect of cold exposure in these cells.
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| Overall design |
Ferrets, male, aged 3 months, were divided into two groups: one group remained at 22 degrees Celsius, while the other group was acclimatized to 4 degrees Celsius for one week. After sacrification, peripheral blood mononuclear cells (PBMC) were isolated and immediately snap frozen in liquid nitrogen. Total RNA was isolated, quantified and qualified, and subsequently used for global gene expression profiling using custom Agilent ferret-specific 2x400K microarrays.
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| Contributor(s) |
Reynés B, van Schothorst EM, Keijer J, Oliver P, Palou A |
| Citation(s) |
31882687 |
| Submission date |
Oct 15, 2014 |
| Last update date |
Jan 08, 2020 |
| Contact name |
Evert M. van Schothorst |
| E-mail(s) |
evert.vanschothorst@wur.nl
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| Organization name |
Wageningen University
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| Lab |
Human and Animal Physiology
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| Street address |
De Elst 1
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| City |
Wageningen |
| ZIP/Postal code |
6708 WD |
| Country |
Netherlands |
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| Platforms (1) |
| GPL19292 |
Agilent-064079 Mustela putorius furo (ferret) array (condensed version) |
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| Samples (10)
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| GSM1525653 |
PBMC, Control, replicate 4 |
| GSM1525654 |
PBMC, Control, replicate 5 |
| GSM1525655 |
PBMC, Control, replicate 6 |
| GSM1525656 |
PBMC, Cold, replicate 1 |
| GSM1525657 |
PBMC, Cold, replicate 2 |
| GSM1525658 |
PBMC, Cold, replicate 3 |
| GSM1525659 |
PBMC, Cold, replicate 4 |
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| Relations |
| BioProject |
PRJNA263901 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE62352_RAW.tar |
422.5 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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