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| Status |
Public on Jun 10, 2019 |
| Title |
No effect of calcifediol supplementation on skeletal muscle transcriptome in vitamin D deficient frail older adults. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Vitamin D deficiency is common among older adults and has been linked to muscle weakness. Vitamin D supplementation has been proposed as a strategy to improve muscle function in older adults. The aim of this study was to investigate the effect of calcifediol (25-hydroxycholecalciferol) on whole genome gene expression in skeletal muscle of vitamin D deficient frail older adults. A double-blind placebo controlled trial was conducted in vitamin D deficient frail older adults (aged above 65), characterized by blood 25-hydroxycholecalciferol concentrations between 20 and 50 nmol/L. Subjects were randomized across the placebo group (n=12) and the calcifediol group (n=10, 10 µg per day). Muscle biopsies were obtained before and after six months of calcifediol or placebo supplementation and subjected to whole genome gene expression profiling using Affymetrix HuGene 2.1ST arrays. Expression of the vitamin D receptor gene was virtually undetectable in human skeletal muscle biopsies. Calcifediol supplementation led to a significant increase in blood 25-hydroxycholecalciferol levels compared to the placebo group. No difference between treatment groups was observed on strength outcomes. The whole transcriptome effects of calcifediol and placebo were very weak. Correcting for multiple testing using false discovery rate did not yield any differentially expressed genes using any sensible cut-offs. P-values were uniformly distributed across all genes, suggesting that low p-values are likely to be false positives. Partial least squares-discriminant analysis and principle component analysis was unable to separate treatment groups. Calcifediol supplementation did not affect the skeletal muscle transcriptome in frail older adults. Our findings indicate that vitamin D supplementation has no effects on skeletal muscle gene expression, suggesting that skeletal muscle may not be a direct target of vitamin D in older adults.
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| Overall design |
Microarray analysis was performed on skeletal muscle biopsies (m. vastus lateralis) from vitamin D deficient frail older adults before and after supplementation with 25-hydroxycholecalciferol.
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| Contributor(s) |
Hangelbroek RW, Vaes AM, Boekschoten MV, Hooiveld GJ, Kersten S |
| Citation(s) |
31138136 |
| Submission date |
Dec 17, 2018 |
| Last update date |
Jun 10, 2019 |
| Contact name |
Guido Hooiveld |
| E-mail(s) |
guido.hooiveld@wur.nl
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| Organization name |
Wageningen University
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| Department |
Div. Human Nutrition & Health
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| Lab |
Nutrition, Metabolism & Genomics Group
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| Street address |
HELIX, Stippeneng 4
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| City |
Wageningen |
| ZIP/Postal code |
NL-6708WE |
| Country |
Netherlands |
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| Platforms (1) |
| GPL17692 |
[HuGene-2_1-st] Affymetrix Human Gene 2.1 ST Array [transcript (gene) version] |
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| Samples (44)
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| GSM3518336 |
skeletal muscle, 25(OH)D3 intervention, baseline sample, subject 3087 |
| GSM3518337 |
skeletal muscle, 25(OH)D3 intervention, intervention sample, subject 3087 |
| GSM3518338 |
skeletal muscle, 25(OH)D3 intervention, baseline sample, subject 3088 |
| GSM3518339 |
skeletal muscle, 25(OH)D3 intervention, intervention sample, subject 3088 |
| GSM3518340 |
skeletal muscle, 25(OH)D3 intervention, baseline sample, subject 3090 |
| GSM3518341 |
skeletal muscle, 25(OH)D3 intervention, intervention sample, subject 3090 |
| GSM3518342 |
skeletal muscle, 25(OH)D3 intervention, baseline sample, subject 3106 |
| GSM3518343 |
skeletal muscle, 25(OH)D3 intervention, intervention sample, subject 3106 |
| GSM3518344 |
skeletal muscle, placebo intervention, baseline sample, subject 3178 |
| GSM3518345 |
skeletal muscle, placebo intervention, intervention sample, subject 3178 |
| GSM3518346 |
skeletal muscle, placebo intervention, baseline sample, subject 3241 |
| GSM3518347 |
skeletal muscle, placebo intervention, intervention sample, subject 3241 |
| GSM3518348 |
skeletal muscle, placebo intervention, baseline sample, subject 3258 |
| GSM3518349 |
skeletal muscle, placebo intervention, intervention sample, subject 3258 |
| GSM3518350 |
skeletal muscle, 25(OH)D3 intervention, baseline sample, subject 3279 |
| GSM3518351 |
skeletal muscle, 25(OH)D3 intervention, intervention sample, subject 3279 |
| GSM3518352 |
skeletal muscle, placebo intervention, baseline sample, subject 3283 |
| GSM3518353 |
skeletal muscle, placebo intervention, intervention sample, subject 3283 |
| GSM3518354 |
skeletal muscle, 25(OH)D3 intervention, baseline sample, subject 3295 |
| GSM3518355 |
skeletal muscle, 25(OH)D3 intervention, intervention sample, subject 3295 |
| GSM3518356 |
skeletal muscle, 25(OH)D3 intervention, baseline sample, subject 3322 |
| GSM3518357 |
skeletal muscle, 25(OH)D3 intervention, intervention sample, subject 3322 |
| GSM3518358 |
skeletal muscle, placebo intervention, baseline sample, subject 3341 |
| GSM3518359 |
skeletal muscle, placebo intervention, intervention sample, subject 3341 |
| GSM3518360 |
skeletal muscle, placebo intervention, baseline sample, subject 3360 |
| GSM3518361 |
skeletal muscle, placebo intervention, intervention sample, subject 3360 |
| GSM3518362 |
skeletal muscle, placebo intervention, baseline sample, subject 3361 |
| GSM3518363 |
skeletal muscle, placebo intervention, intervention sample, subject 3361 |
| GSM3518364 |
skeletal muscle, placebo intervention, baseline sample, subject 3375 |
| GSM3518365 |
skeletal muscle, placebo intervention, intervention sample, subject 3375 |
| GSM3518366 |
skeletal muscle, placebo intervention, baseline sample, subject 3410 |
| GSM3518367 |
skeletal muscle, placebo intervention, intervention sample, subject 3410 |
| GSM3518368 |
skeletal muscle, placebo intervention, baseline sample, subject 3430 |
| GSM3518369 |
skeletal muscle, placebo intervention, intervention sample, subject 3430 |
| GSM3518370 |
skeletal muscle, 25(OH)D3 intervention, baseline sample, subject 3498 |
| GSM3518371 |
skeletal muscle, 25(OH)D3 intervention, intervention sample, subject 3498 |
| GSM3518372 |
skeletal muscle, 25(OH)D3 intervention, baseline sample, subject 3516 |
| GSM3518373 |
skeletal muscle, 25(OH)D3 intervention, intervention sample, subject 3516 |
| GSM3518374 |
skeletal muscle, placebo intervention, baseline sample, subject 3614 |
| GSM3518375 |
skeletal muscle, placebo intervention, intervention sample, subject 3614 |
| GSM3518376 |
skeletal muscle, 25(OH)D3 intervention, baseline sample, subject 3695 |
| GSM3518377 |
skeletal muscle, 25(OH)D3 intervention, intervention sample, subject 3695 |
| GSM3518378 |
skeletal muscle, placebo intervention, baseline sample, subject 3731 |
| GSM3518379 |
skeletal muscle, placebo intervention, intervention sample, subject 3731 |
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| Relations |
| BioProject |
PRJNA510436 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE123993_RAW.tar |
207.8 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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