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Series GSE117026 Query DataSets for GSE117026
Status Public on Jul 13, 2018
Title Marginal selenium deficiency down-regulates inflammation-related genes in splenic leukocytes of the mouse
Organism Mus musculus
Experiment type Expression profiling by array
Summary Moderate selenium deficiency may lead to an impaired capacity to cope with health challenges. Functional effects of suboptimal selenium intake are not fully known, and biomarkers for an insufficient selenium supply are inadequate. We therefore fed mice diets of moderately deficient or adequate selenium intake for 6 weeks. Changes in global gene expression were monitored by microarray analysis in splenic leukocytes. Genes for four selenoproteins, Sepw1, Gpx1, Selh and Sep15, were the most significantly down-regulated in moderate selenium deficiency, and this was confirmed by quantitative polymerase chain reaction (qPCR). Classification of significantly affected genes revealed that processes related to inflammation, heme biosynthesis, DNA replication and transcription, cell cycle and transport were affected by selenium restriction. Down-regulation by moderate selenium deficiency of specific genes involved in inflammation and heme biosynthesis was confirmed by qPCR. Myeloperoxidase and lysozyme activities were decreased in selenium-restricted leukocytes, providing evidence for functional consequences. Genes for 31 nuclear factor (NF)-κB targets were down-regulated in moderate selenium deficiency, indicating an impaired NF-κB signaling. Together, the observed changes point to a disturbance in inflammatory response. The selenoproteins found here to be sensitive to selenium intake in murine leukocytes might also be useful as biomarkers for a moderate selenium deficiency in humans.
 
Overall design Male C57BL/6J mice (3–4 wk of age) from Charles River (Sulzfeld, Germany) were randomly assigned to the selenium-deficient or selenium-adequate group (12 mice per group) with free access to food and water. The selenium-adequate diet (Se-adeq) was produced by mixing selenomethionine (Acros, Geel, Belgium) into the selenium-deficient diet (Se-def; No. C1045 with 50% carbohydrates, 17% protein, 5% fat, 4% fibre, and mixture of micronutrients; Altromin, Lage, Germany) containing 0.086mg Se/kg (Riese et al., Endocrinology 2006) to yield a selenium content of 0.15 mg/kg corresponding to the dietary reference intake for mice. Diets were fed as powder for 6wk until mice were killed in the non-fasted state. Animals were anesthetized with isofluran and blood with heparinized capillaries by puncture of the retroorbital plexus. Anesthetized animals were killed by cervical dislocation. Spleens were removed aseptically, placed on a sterile microscope slide and crushed with the end of a 6-ml syringe plunger. Released cells were diluted in 5ml of cell culture medium (RPMI with 5% fetal calf serum; Gibco, Karlsruhe, Germany). Clumps were dispersed by drawing and expelling the suspension repeatedly through a 6-ml syringe with a 20-gauge needle. A 100-μmmesh was used to remove clumps and particles and to receive a single cell suspension. The mesh was rinsed with 5 ml RPMI. After centrifugation (5 min, 200g) and washing with 10 ml RPMI, erythrocytes were lysed for 5 min in 5 ml of ammonium chloride lysis buffer (0.15 mol/L NH4Cl, 10 mmol/L KHCO3, 0.1 mmol/L Na2EDTA, pH 7.4). Thereafter, 15 ml of balanced salt solution containing 0.2% bovine serum albumin was added, and the suspension was centrifuged for 5 min at 200g. The pellet was suspended in 1 ml phosphate-buffered saline (PBS) and transferred into a 2-ml tube. Thirty microliters of the suspension was smeared on microscope slides and dried on air for counting. The cells of the remaining suspension were pelleted (5 min, 200g) and washed with 1 ml PBS. The pellet was frozen at−80°C until RNA isolation. As determined with May–Grünwald–Giemsa staining, the leukocyte population was independent of the selenium status and on average was composed of 79% lymphocytes, 18% granulocytes and 2% monocytes.
 
Contributor(s) Kipp A, Banning A, Brigelius-Flohe R, Keijer J, van Schothorst EM
Citation(s) 22137268
Submission date Jul 12, 2018
Last update date Jul 15, 2018
Contact name Evert M. van Schothorst
E-mail(s) evert.vanschothorst@wur.nl
Organization name Wageningen University
Lab Human and Animal Physiology
Street address De Elst 1
City Wageningen
ZIP/Postal code 6708 WD
Country Netherlands
 
Platforms (1)
GPL7042 Agilent-012694 Whole Mouse Genome G4122A (Probe Name version)
Samples (24)
GSM3267469 leukocytes_Se-def_replicate 01
GSM3267470 leukocytes_Se-def_replicate 02
GSM3267471 leukocytes_Se-def_replicate 03
This SubSeries is part of SuperSeries:
GSE117027 Expression profiling of selenium deficiency in mouse colon and splenic leukocytes
Relations
BioProject PRJNA480872

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE117026_RAW.tar 92.1 Mb (http)(custom) TAR (of TXT)
GSE117026_normalized_data.txt.gz 1.6 Mb (ftp)(http) TXT
Processed data are available on Series record

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