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Series GSE117025 Query DataSets for GSE117025
Status Public on Jul 13, 2018
Title Four selenoproteins, protein biosynthesis, and Wnt signalling are particularly sensitive to limited selenium intake in mouse colon
Organism Mus musculus
Experiment type Expression profiling by array
Summary Selenium is an essential micronutrient. Its recommended daily allowance is not attained by a significant proportion of the population in many countries and its intake has been suggested to affect colorectal carcinogenesis. Therefore, microarrays were used to determine how both selenoprotein and global gene expression patterns in the mouse colon were affected by marginal selenium deficiency comparable to variations in human dietary intakes. Two groups of 12 mice each were fed a selenium-deficient (0.086mg Se/kg) or a selenium-adequate (0.15mg Se/kg) diet. After 6wk, plasma selenium level, liver, and colon glutathione peroxidase (GPx) activity in the deficient group was 12, 34, and 50%, respectively, of that of the adequate group. Differential gene expression was analysed with mouse 44K whole genome microarrays. Pathway analysis by GenMAPP identified the protein biosynthesis pathway as most significantly affected, followed by inflammation, Delta-Notch and Wnt pathways. Selected gene expression changes were confirmed by quantitative real-time PCR. GPx1 and the selenoproteins W, H, and M, responded significantly to selenium intake making them candidates as biomarkers for selenium status. Thus, feeding a marginal selenium-deficient diet resulted in distinct changes in global gene expression in the mouse colon. Modulation of cancer-related pathways may contribute to the higher susceptibility to colon carcinogenesis in low selenium status.
 
Overall design Male C57BL/6J mice (3–4 wk of age) from Charles River (Sulzfeld, Germany) were randomly assigned to the selenium-deficient or selenium-adequate group (12 mice per group) with free access to food and water. The selenium-adequate diet (Se-adeq) was produced by mixing selenomethionine (Acros, Geel, Belgium) into the selenium-deficient diet (Se-def; No. C1045 with 50% carbohydrates, 17% protein, 5% fat, 4% fibre, and mixture of micronutrients; Altromin, Lage, Germany) containing 0.086mg Se/kg (Riese et al., Endocrinology 2006) to yield a selenium content of 0.15 mg/kg corresponding to the dietary reference intake for mice. Diets were fed as powder for 6wk until mice were killed in the non-fasted state. Animals were anesthetized with isofluran and blood was withdrawn from the retro-orbital plexus. Anesthetized animals were killed by cervical dislocation. Plasma and tissues freeze clamped in liquid nitrogen were stored at -80C.
 
Contributor(s) Kipp A, Banning A, Brigelius-Flohe R, Keijer J, van Schothorst EM
Citation(s) 19810021
Submission date Jul 12, 2018
Last update date Jul 15, 2018
Contact name Evert M. van Schothorst
E-mail(s) evert.vanschothorst@wur.nl
Organization name Wageningen University
Lab Human and Animal Physiology
Street address De Elst 1
City Wageningen
ZIP/Postal code 6708 WD
Country Netherlands
 
Platforms (1)
GPL7042 Agilent-012694 Whole Mouse Genome G4122A (Probe Name version)
Samples (24)
GSM3267445 colon_Se-def_replicate 01
GSM3267446 colon_Se-def_replicate 02
GSM3267447 colon_Se-adeq_replicate 01
This SubSeries is part of SuperSeries:
GSE117027 Expression profiling of selenium deficiency in mouse colon and splenic leukocytes
Relations
BioProject PRJNA480871

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE117025_RAW.tar 92.7 Mb (http)(custom) TAR (of TXT)
GSE117025_normalized_data.txt.gz 2.0 Mb (ftp)(http) TXT
Processed data are available on Series record

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