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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Feb 10, 2018 |
| Title |
The Peroxisome Proliferator-Activated Receptor α is dispensable for cold-induced adipose tissue browning in mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Chronic cold exposure causes white adipose tissue (WAT) to adopt features of brown adipose tissue, a process known as browning. Previous studies have hinted at a possible role for the transcription factor Peroxisome Proliferator-Activated Receptor alpha (PPARα) in cold-induced browning. Here we aimed to investigate the importance of PPARα in driving transcriptional changes during cold-induced browning in mice. Male wildtype and PPARα−/− mice were housed at thermoneutrality (28 °C) or cold (5 °C) for 10 days. Whole genome expression analysis was performed on inguinal WAT. In addition, other analyses were carried out. Whole genome expression data of livers of wildtype and PPARα−/− mice fasted for 24 h served as positive control for PPARα-dependent gene regulation.Cold exposure increased food intake and decreased weight of BAT and WAT to a similar extent in wildtype and PPARα−/− mice. Except for plasma non-esterified fatty acids, none of the cold-induced changes in plasma metabolites were dependent on PPARα genotype. Histological analysis of inguinal WAT showed clear browning upon cold exposure but did not reveal any morphological differences between wildtype and PPARα−/− mice. Transcriptomics analysis of inguinal WAT showed a marked effect of cold on overall gene expression, as revealed by principle component analysis and hierarchical clustering. However, wildtype and PPARα−/− mice clustered together, even after cold exposure, indicating a similar overall gene expression profile in the two genotypes. Pathway analysis revealed that cold upregulated pathways involved in energy usage, oxidative phosphorylation, and fatty acid β-oxidation to a similar extent in wildtype and PPARα−/− mice. Furthermore, cold-mediated induction of genes related to thermogenesis such as Ucp1, Elovl3, Cox7a1, Cox8, and Cidea, as well as many PPAR target genes, was similar in wildtype and PPARα−/− mice. Finally, pharmacological PPARα activation had a minimal effect on expression of cold-induced genes in murine WAT.Cold-induced changes in gene expression in inguinal WAT are unaltered in mice lacking PPARα, indicating that PPARα is dispensable for cold-induced browning.
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| Overall design |
Wild type and PPARα-null mice were housed at thermoneutrality (28 °C) or cold (5 °C) for 10 days whereafter inguinal white adipose tissue was subjected to gene expression profiling.
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| Contributor(s) |
Defour M, Dijk W, Ruppert P, Nascimento E, Schrauwen P, Kersten S |
| Citation(s) |
29455954 |
| Submission date |
Feb 09, 2018 |
| Last update date |
May 14, 2018 |
| Contact name |
Guido Hooiveld |
| E-mail(s) |
guido.hooiveld@wur.nl
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| Organization name |
Wageningen University
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| Department |
Div. Human Nutrition & Health
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| Lab |
Nutrition, Metabolism & Genomics Group
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| Street address |
HELIX, Stippeneng 4
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| City |
Wageningen |
| ZIP/Postal code |
NL-6708WE |
| Country |
Netherlands |
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| Platforms (1) |
| GPL17400 |
[MoGene-2_1-st] Affymetrix Mouse Gene 2.1 ST Array [transcript (gene) version] |
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| Samples (22)
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| GSM2991102 |
Inguinal WAT, cold exposure, PPARα knockout mouse, replicate 1 |
| GSM2991103 |
Inguinal WAT, cold exposure, PPARα knockout mouse, replicate 2 |
| GSM2991104 |
Inguinal WAT, cold exposure, PPARα knockout mouse, replicate 3 |
| GSM2991105 |
Inguinal WAT, cold exposure, PPARα knockout mouse, replicate 4 |
| GSM2991106 |
Inguinal WAT, cold exposure, PPARα knockout mouse, replicate 5 |
| GSM2991107 |
Inguinal WAT, cold exposure, wild type mouse, replicate 1 |
| GSM2991108 |
Inguinal WAT, cold exposure, wild type mouse, replicate 2 |
| GSM2991109 |
Inguinal WAT, cold exposure, wild type mouse, replicate 3 |
| GSM2991110 |
Inguinal WAT, cold exposure, wild type mouse, replicate 4 |
| GSM2991111 |
Inguinal WAT, cold exposure, wild type mouse, replicate 5 |
| GSM2991112 |
Inguinal WAT, thermoneutrality, PPARα knockout mouse, replicate 1 |
| GSM2991113 |
Inguinal WAT, thermoneutrality, PPARα knockout mouse, replicate 2 |
| GSM2991114 |
Inguinal WAT, thermoneutrality, PPARα knockout mouse, replicate 3 |
| GSM2991115 |
Inguinal WAT, thermoneutrality, PPARα knockout mouse, replicate 4 |
| GSM2991116 |
Inguinal WAT, thermoneutrality, PPARα knockout mouse, replicate 5 |
| GSM2991117 |
Inguinal WAT, thermoneutrality, PPARα knockout mouse, replicate 6 |
| GSM2991118 |
Inguinal WAT, thermoneutrality, wild type mouse, replicate 1 |
| GSM2991119 |
Inguinal WAT, thermoneutrality, wild type mouse, replicate 2 |
| GSM2991120 |
Inguinal WAT, thermoneutrality, wild type mouse, replicate 3 |
| GSM2991121 |
Inguinal WAT, thermoneutrality, wild type mouse, replicate 4 |
| GSM2991122 |
Inguinal WAT, thermoneutrality, wild type mouse, replicate 5 |
| GSM2991123 |
Inguinal WAT, thermoneutrality, wild type mouse, replicate 6 |
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| Relations |
| BioProject |
PRJNA433669 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE110420_RAW.tar |
108.6 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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