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Series GSE32706 Query DataSets for GSE32706
Status Public on Oct 08, 2011
Title Comparative transcriptomics and metabolomic analysis of fenofibrate and fish oil treatments in mice
Organism Mus musculus
Experiment type Expression profiling by array
Summary Elevated circulating triglycerides, which are considered a risk factor for cardiovascular disease, can be targeted by treatment with fenofibrate or fish oil. To gain insight into underlying mechanisms, we carried out a comparative transcriptomics and metabolomics analysis of the effect of 2 week treatment withfenofibrate and fish oil in mice. Plasma triglycerides were significantly decreased byfenofibrate (-49.1%) and fish oil (-21.8%), whereas plasma cholesterol was increased by fenofibrate (+29.9%) and decreased by fish oil (-32.8%). Levels of various phospholipid species were specifically decreased by fish oil, while levels of Krebs cycle intermediates were increased specifically by fenofibrate. Plasma levels of many amino acids were altered by fenofibrate and to a lesser extent by fish oil. Both fenofibrate and fish oil upregulated genes involved in fatty acid metabolism, and downregulated genes involved in blood coagulation and fibrinolysis. Significant overlap in gene regulation by fenofibrate and fish oil was observed, reflecting their property as high or low affinity agonist for PPARα, respectively. Fenofibrate specifically downregulated genes involved in complement cascade and inflammatory response. Fish oil specifically downregulated genes involved in cholesterol and fatty acid biosynthesis, and upregulated genes involved in amino acid and arachidonic acid metabolism. Taken together, the data indicate that despite being similarly potent towards modulating plasma free fatty acids, cholesterol and triglyceride levels, fish oil causes modest changes in gene expression likely via activation of multiple mechanistic pathways, whereas fenofibrate causes pronounced gene expression changes via a single pathway, reflecting the key difference between nutritional and pharmacological intervention.
 
Overall design Expression profiling of liver from mice fed control diet, fish oil or fenofibrate for 2 weeks.
 
Contributor(s) Lu Y, Boekschoten MV, Wopereis S, Müller M, Kersten S
Citation(s) 21954454
Submission date Oct 08, 2011
Last update date Feb 11, 2019
Contact name Guido Hooiveld
E-mail(s) guido.hooiveld@wur.nl
Organization name Wageningen University
Department Div. Human Nutrition & Health
Lab Nutrition, Metabolism & Genomics Group
Street address HELIX, Stippeneng 4
City Wageningen
ZIP/Postal code NL-6708WE
Country Netherlands
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (33)
GSM812576 liver_fishoil_replicate1
GSM812577 liver_fishoil_replicate2
GSM812578 liver_control_replicate1
Relations
BioProject PRJNA147003

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Supplementary file Size Download File type/resource
GSE32706_RAW.tar 123.6 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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