Elsevier

Journal of Virological Methods

Volume 248, October 2017, Pages 26-30
Journal of Virological Methods

Short communication
A novel highly sensitive, rapid and safe Rift Valley fever virus neutralization test

https://doi.org/10.1016/j.jviromet.2017.06.001Get rights and content
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open access

Abstract

Antibodies specific for Rift Valley fever virus (RVFV) can be detected by diverse methods, including ezyme-linked immunosortbent assay (ELISA) and virus neutralization test (VNT). The VNT is superior in sensitivity and specificity and is therefore considered the gold standard serological assay. Classical VNTs make use of virulent RVFV and therefore have to be performed in biosafety level 3 laboratories. Here, we report the development of a novel VNT that is based on an avirulent RVFV expressing the enhanced green fluorescent protein (eGFP), which can be performed safely outside level 3 biocontainment facilities. Evaluation with a broad panel of experimental sera and field sera demonstrated that this novel VNT is faster and more sensitive than the classical VNT.

Keywords

Rift Valley fever virus
4-Segmented
Virus neutralization test
GFP
Ruminants

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