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Trichomes: interaction sites of tomato leaves with biotrophic powdery mildew pathogens

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Abstract

The present study aimed to explore the possibility of using the type I trichomes of tomato (Solanum lycopersicum) to monitor the infection processes of powdery mildews by microscopy. Individual trichome cells of two tomato genotypes were inoculated with pathogenic and non-pathogenic powdery mildew species, Pseudoidium neolycopersici, Erysiphe trifoliorum and Podosphaera xanthii. On the trichome cells of the tomato cultivar Moneymaker, hyphae of the pathogenic Pseudoidium neolycopersici (isolates KTP-03 and KTP-04) grew vigorously; whereas hyphal growth of the non-pathogenic Erysiphe trifoliorum and Podosphaera xanthii ceased after appressorium formation, which was associated with papilla formation and hypersensitive cell death, respectively. Similar infection processes of the tested powdery mildews were seen in Moneymaker epidermal cells. Therefore, tomato trichomes are suitable for analysing, at individual cell level, the infection processes of different pathotypes of powdery mildews and for observing the cytological responses of plants by non-pathogenic powdery mildews. On the other hand, it was observed that both isolates KTP-03 and KTP-04 failed to produce conidiophores on the hyphae elongating on Moneymaker trichomes. Similarly, no conidiophores were produced on the hyphae elongating on trichomes of Solanum peruvianum LA2172, which is resistant to KTP-03 and susceptible to KTP-04. Interestingly, delayed cell death occurred in LA2172 epidermal cells, which were attacked by KTP-03 hyphae elongating from trichomes and conidiophores were formed on new hyphae growing from the leaf epidermal cells. Thus, leaf trichomes and epidermal cells of the wild tomato species LA2172 reacted differently to the avirulent isolate KTP-03.

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Acknowledgements

This work was supported by a Grant for Scientific Research from Faculty of Agriculture, Kindai University.

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Authors and Affiliations

  1. Department of Chemical Biological Sciences, Faculty of Science, Japan Women’s University, Tokyo, 112-8681, Japan

    Tomoko Suzuki

  2. Laboratory of Phytoprotection, Science and Technology, Faculty of Agriculture, Kindai University, Nara, 631-8505, Japan

    Tomoe Murakami, Yoshihiro Takizumi, Hiroyuki Ishimaru, Daiki Kudo, Yoshinori Matsuda & Teruo Nonomura

  3. Plant Center, Institute of Advanced Technology, Kindai University, Wakayama, 644-0025, Japan

    Yoshihiro Takikawa

  4. Pharmaceutical Research and Technology Institute, Kindai University, Osaka, 577-8502, Japan

    Koji Kakutani

  5. Plant Breeding, Wageningen University & Research, Droevendaalsesteeg 1, 6708PB, Wageningen, the Netherlands

    Yuling Bai

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  1. Tomoko Suzuki
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  2. Tomoe Murakami
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  3. Yoshihiro Takizumi
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  4. Hiroyuki Ishimaru
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  5. Daiki Kudo
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  9. Yuling Bai
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  10. Teruo Nonomura
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Correspondence to Yuling Bai or Teruo Nonomura.

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Suzuki, T., Murakami, T., Takizumi, Y. et al. Trichomes: interaction sites of tomato leaves with biotrophic powdery mildew pathogens. Eur J Plant Pathol 150, 115–125 (2018). https://doi.org/10.1007/s10658-017-1257-y

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  • DOI: https://doi.org/10.1007/s10658-017-1257-y

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