Abstract
Plant roots have a profound effect on soil microbial activity, particularly in the rhizosphere. Hence, it is important to understand the potential effects of genetically modified (GM) crops on soil microbial activity and related processes such as litter decomposition. In this study, we compared the effects of GM potato Modena on soil microbial activity and carbon (C) and nitrogen (N) mineralization to effects induced by Modena’s parental isoline (Karnico) and a conventional potato cultivar (Aventra). A field experiment was conducted at two sites to assess microbial catabolic diversity (using MicroRespTM) in the rhizosphere and in bulk soil, during flowering and senescence of the potato plants. In a laboratory experiment with soil and potato litter from the field experiment, we investigated whether the cultivars had modified the activity of soil microbial communities to such an extent that this affected C and N mineralization. Results of the field experiment showed no GM-induced effects on microbial catabolic diversity, while effects of field site location and sampling date were significant. Multivariate analysis including plant traits and soil characteristics revealed that microbial catabolic activities in rhizosphere soil were strongly correlated with soil organic matter and tuber sucrose content, whereas in bulk soil, they were primarily correlated with soil moisture. In the laboratory experiment, we found that Modena induced a “home-field advantage” in N mineralization, yet this effect was inconsistent across locations and was also observed for other cultivars. Based on our data and results from previous studies, we conclude that the effects of GM cultivar Modena on soil microbial activity and litter decomposition fall within the normal range of effects found for conventional potato cultivars.
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Introduction
Since the commercial introduction of genetically modified (GM) crops in 1996, the total area grown nowadays with GM varieties has increased to 175 million ha, with two thirds of this area located in the Americas (James 2013). In the Netherlands, GM crops are not yet grown commercially due to concerns about possible environmental side effects (e.g., Bruinsma et al. 2003). Depending on the nature of their genetic modification, GM plants could affect soil ecosystems through trait-mediated shifts in root physiology, root exudates, and/or litter quality (Powell 2007). Hence, during the growing season, the effects of GM crops on soil biota are most likely to occur in the rhizosphere, a thin zone around the roots and a hotspot for soil microorganisms (Hartmann et al. 2008). In terms of risk assessment, it is therefore relevant to know whether GM plant-induced changes in the rhizosphere could have prolonged effects on soil biota (Mulder et al. 2006; Powell 2007; Hannula et al. 2014).
Current molecular techniques allow commercial development of marker-free GM crops, i.e., cultivars without plant transformation markers such as kanamycin resistance (e.g., Tuteja et al. 2012). Normally, potato (Solanum tuberosum) tubers contain starch consisting of amylose and amylopectin in roughly a 1:5 ratio (Broothaerts et al. 2007), but the cultivar Modena (BASF GmbH, Limburgerhof, Germany) produces tubers with amylose-free starch. This genetic modification was accomplished by inhibiting the transcription of the granule-bound starch synthase gene, which is essential for amylose production, in the parental cultivar Karnico. The resulting amylose-free starch in Modena tubers is an attractive ingredient for a range of industrial applications (Visser and Jacobsen 1993). Since this particular trait involves a direct inhibition of a specific pathway in carbohydrate synthesis and plant transformation markers are absent (De Vetten et al. 2003), any environmental effects of this GM crop may be ascribed to its altered carbohydrate metabolism.
Several studies have assessed the effects of Modena (and similar potato genotypes with genetically modified starch content) on the soil microbial community composition (e.g., Milling et al. 2005; Hannula et al. 2010). The effects of Modena on soil biota were mainly detected in the rhizosphere and during plant senescence, with the effects assumed to be caused by changed root exudation patterns (İnceoğlu et al. 2013). Dias et al. (2013) observed differences between the rhizosphere microbial communities of Modena versus Karnico, in terms of the relative proportions of the gram-negative bacteria Pseudomonas and Burkholderia. However, several studies also showed that plant growth stage, soil type, and yearly fluctuations in weather conditions had more prominent effects on rhizosphere biota, than GM versus conventional potato cultivars (Gschwendtner et al. 2011; Hannula et al. 2012a, b; İnceoğlu et al. 2012, 2013).
The genetically modified carbohydrate metabolism of Modena may not only lead to distinct root exudation patterns but may also affect the biochemical properties of above- and belowground plant parts, resulting in altered litter quality (Jacobsen et al. 1989; Privalle et al. 2013). In turn, this could affect decomposition processes. For example, Hannula et al. (2013) observed that tubers and leaves of Modena decomposed more rapidly than litter of Karnico, although this difference was only observed in the first month of incubation. Furthermore, due to their altered litter quality and root exudation patterns, GM crops such as Modena could influence C and N mineralization by inducing a “home-field advantage.” This term has been used to describe the effect that plant litter decomposes more rapidly in its “home” soil (the location of the plant producing the litter) than in a different soil, because of local adaptation of the soil biota (Ayres et al. 2009).
The first aim of this study was to compare microbial catabolic diversity in rhizosphere and bulk soil from field crops of Modena (GM cultivar), Karnico (parental line), and Aventra (conventional cultivar) and relate the observed effects to potato traits. Our second aim was to investigate in a laboratory experiment whether Modena influenced soil C and N mineralization to the extent of inducing a home-field advantage in litter decomposition. We included conventional potato cultivars (Aventra and Karnico) in our experiments to assess whether potential GM trait-related effects would fall within the normal range of variation between conventional cultivars (Griffiths et al. 2007; Perry et al. 2009).
Material and methods
Field experiment
The field experiment was performed at two field sites (VMD and BUI), located within 10 km of each other in the province of Drenthe, The Netherlands. Soil at location VMD was characterized as sandy peat and soil at location BUI as loamy sand. Both soils had similar soil pH, but otherwise differed in various soil characteristics, most importantly organic matter content, sand fraction, and average sand particle size (Table 1). Both fields had been cropped with potato in rotation with barley for many decades.
On 27 April 2011, three different potato cultivars (Karnico, Modena, and Aventra) were planted at both field sites. All varieties are used for potato starch production and have a relative high starch content of the tubers (Hannula et al. 2012b). The variety Modena was bred from Karnico (for details, see De Vetten et al. 2003) and has a much lower amylose content as compared to Karnico and Aventra. Potatoes were planted in a randomized complete block design with four replicate plots per cultivar (12 plots per site). Each plot (3.0 × 2.8 m) was planted with 28 potato tubers, divided over four ridges; distance between ridges was 75 cm, and planting distance within ridges was 40 cm. Fertilizer application was based on the standard recommendation for potato crops, adjusted to local soil conditions. At location VMD, potatoes were fertilized with 140 kg N ha−1, 50 kg P2O5 ha−1 (via calcium ammonium nitrate and NP 26-14) and 1500 kg protamylasse ha−1 (AVEBE, Veendam, The Netherlands); at location BUI, with 250 kg N ha−1, 250 kg K2O ha−1 (via calcium ammonium nitrate and Patentkali), and 20,000 kg pig slurry ha−1.
Potato tuber yield was neither affected by potato variety nor by location. Potato tuber starch, sucrose, vitamin C, and glycoalkaloids were extracted and analyzed as described by Privalle et al. (2013). Potato tubers used for the constituent analyses were sampled from trials located next to the experimental fields.
Collection of soil and litter samples
At both field site locations, soil samples were taken during the plant flowering stage (15 August 2011) and senescence stage (13 September 2011), since effects were expected to be most pronounced during these growth stages (e.g., İnceoğlu et al. 2013). In each plot, a composite sample of the bulk soil was taken, consisting of 16 cores (∅ 2 cm, depth 20 cm) taken between plants from each of the four ridges. Rhizosphere samples were taken by carefully digging out potato plants using a spade. Soil still adhering to the roots after shaking the plants twice was considered rhizosphere soil, and was collected with a brush. All samples were immediately cooled after sampling (4 °C). For the litter decomposition experiment in the laboratory (see “Laboratory litter decomposition experiment”), we collected bulk soil and plant shoots from each cultivar plot (at both field site locations) on 13 September 2011. Plant samples were dried (70 °C for 24 h), ground, and analyzed for C and N content using flash combustion in an elemental analyzer (Interscience/Fisons EA 1108, The Netherlands) (Pella and Colombo 1973).
Microbial catabolic diversity
Microbial catabolic diversity was assessed with the MicroRespTM procedure (Campbell et al. 2003), using a selection of C substrates based on plant root exudation patterns (Campbell et al. 1997). Within 24 h after sampling, soil samples were sieved (2-mm mesh) and added to the deepwell plates using the standard filling device to add about 300 μl of soil to each well. Soil dry weight per well was calculated based on the weight of fresh soil added and the soil moisture content of the soil sample (determined by drying at 105 °C for 24 h). Filled plates were not pre-incubated, because this could lead to loss of plant-induced rhizosphere effects (Jones and Darrah 1993). The C substrates were dissolved in demineralized water and prepared as stock solutions designed to achieve a final concentration of either 30 mg C g−1 soil solution (citric acid, glucose, malic acid, sucrose, trehalose, and Tween 80) or 7.5 mg C g−1 soil solution (alanine, arginine, phenylalanine, oxalic acid, and fumaric acid, which have lower solubility), where calculation of the soil solution was based on soil moisture content plus 25 μl added stock solution. Substrate solutions, and water as a control, were dispensed into deepwell plates (four replicate wells per substrate, per soil sample). The absorbance of the indicator dye (a solution of cresol red, potassium chloride, sodium bicarbonate in 3 % purified agar) in the detection plates was measured at 570 nm before and after the incubation period (at 25 °C for 6 h) using a microplate reader (Vmax, Molecular Devices, Sunnyvale, CA, USA). Color development was normalized by subtracting the time zero measurement (averaged per plate) from the measured color development of the dye in each well after 6 h of incubation according to Campbell et al. (2003) and converted to CO2 concentrations using the following calibration curve: %CO2 = 0.002 × A 570 −3.11 (R 2 = 0.93), where %CO2 (vol/vol) is the concentration in the headspace after incubation and A 570 is the normalized absorbance. Median CO2 concentrations (n = 4) for each substrate and plot were converted to respiration rates (μg CO2–C g−1 dry soil h−1), corrected for median respiration rates of the controls (water).
Laboratory litter decomposition experiment
To assess whether the various potato cultivars could induce a home-field advantage in decomposition, we conducted a laboratory experiment using soil and plant litter (shoots) collected from the field experiment (see “Collection of soil and litter samples”). For each cultivar plot, four bottles (333 ml) were filled each with 75 g soil (fresh weight) collected from the respective plot on 13 September 2011 (12 plots per site including 4 replicates per cultivar, 2 sites, resulting in 96 bottles). In each of those sets, one bottle was amended with 1.5 g dried and ground potato shoot litter from the cultivar grown in the same plot as from which the soil was collected (home situation); two bottles received 1.5 g potato shoot material from one of the other cultivars, grown in other plots within the same experimental block (away situation); and one bottle was kept as a soil-only control (no litter added). The plant material was mixed into the soil, and all bottles were incubated during 121 days in the dark at 20 °C. Soil moisture content was kept at 60 % water holding capacity throughout the experiment.
Carbon mineralization was determined in all bottles, based on CO2 production measured on days 14, 17, 23, 37, 65, and 121 of the incubation. On these days, each bottle was flushed with compressed air for 15 min, closed with an air-tight lid, and incubated for 4 h at 20 °C in the dark; after which, the CO2 concentration in the headspace was measured with a Photoacoustic Field Gas-Monitor (INNOVA 1412, LumaSenseTM Technologies, Denmark). C mineralization rates (μg CO2 g−1 dry soil h−1) in soil-litter mixtures were corrected for rates in soil-only incubations. For each replicate cultivar plot, the area under the curve for total net C mineralization over the 121-day incubation period was calculated (Fig. 3).
Home-field advantage was calculated for net C and N mineralization and net change in microbial biomass N over the 121-day incubation period, based on the equations used by Ayres et al. (2009):
where ADH is the additional decomposition at home for each cultivar; i, j, and k represent potato shoot litter from Karnico, Modena, and Aventra, respectively; I, J, and K are soil samples from the plots where the potato cultivars Karnico, Modena, and Aventra were grown, respectively; D is a measure of decomposition (net C or N mineralization, or net change in microbial biomass N, over the 121-day incubation period); HDD and ADD represent home decomposition difference and away decomposition difference, respectively; H represents the total home-field advantage for all cultivars combined; and N represents the number of cultivars.
Microbial biomass N was determined on day 0 (in bulk soil samples from each cultivar plot) and day 121 (in all bottles) according to the chloroform fumigation and extraction technique, using 0.5 M K2SO4 as extractant (1:4 w/v, fresh weight basis) (k EC = 0.54) (Brookes et al. 1985). Total dissolved N from the non-fumigated subsamples was used to determine the net N mineralization. Soil mineral N content was measured on day 0 and day 121 using a segmented flow analyzer (Skalar Analytical, B.V. Breda, The Netherlands). Net change in microbial biomass N and net N mineralization in soil-litter incubations over 121 days were corrected for values in soil-only controls.
Data analysis
In the field experiment, the effects of cultivar, field site location, and sampling date on microbial catabolic activities in the rhizosphere and bulk soil were assessed through redundancy analysis (RDA) in Canoco v. 5.0 (Van Dobben et al. 1999). The significance of correlations between variables was tested using Monte Carlo permutation tests (999 random permutations). As we detected relatively strong effects of field site location and sampling date, we conducted a follow-up RDA analysis including soil characteristics (soil moisture, organic matter) and potato tuber contents (glycoalkaloids, starch, sucrose, and vitamin C) as explanatory variables. In this RDA analysis, we used a forward (i.e., stepwise) selection of explanatory variables to select the variables comprising the strongest effects and to sort out variance already explained by previously selected variables. Block effects were partialled out by entering block indicator variables as covariables. Explanatory variables showing significant correlations (p ≤ 0.05) with microbial catabolic diversity (response to specific substrates) were included in ordination plots (Fig. 1).
In the soil-litter incubation experiment, ANOVA was used to analyze the effects of cultivar, field site location (BUI versus VMD), and the interaction between these factors on decomposition-related variables (C and N mineralization and microbial biomass N). Data from the soil-only controls (basal decomposition) were analyzed separately; ANOVA of decomposition variables in soil-litter mixtures focussed on home situations (i.e., treatments where soil and litter came from one and the same plot). An additional ANOVA was conducted to analyze cultivar and field site location effects on the C:N ratio of potato shoot litter.
To assess whether the different potato cultivars induced a home-field advantage in decomposition, we calculated the average and 95 % confidence interval (average ± 1.96 × SE) for the additional decomposition at home (ADH; see “Laboratory litter decomposition experiment”) for net C and N mineralization and net change in microbial biomass N over the 121-day incubation period, assuming a normal distribution of the data. Where the 95 % confidence interval of ADH did not include zero, we considered the home-field advantage (ADH > 0) or home-field disadvantage (ADH < 0) to be significant.
Results
Field experiment: microbial catabolic diversity in rhizosphere and bulk soil
In the field experiment, microbial catabolic activity was not significantly affected by potato cultivar, neither in rhizosphere nor bulk soil. Instead, RDA detected significant effects of field site location and sampling date, both in rhizosphere soil (location effect 67.3 % explained variance, F = 85.2, p < 0.002; sampling date effect 4.8 % explained variance, F = 3.1, p = 0.02) and in bulk soil (location effect 53.3 % explained variance, F = 47.8, p < 0.002; sampling date effect 16.1 % explained variance, F = 8.9, p < 0.002).
The follow-up redundancy analysis, including specific cultivar traits and soil characteristics as explanatory variables, showed that microbial catabolic activities in rhizosphere soil were most strongly correlated with soil organic matter and tuber sucrose content, whereas in bulk soil, they were primarily correlated with soil moisture (Table 2). Specifically, in rhizosphere soil, microbial response to sucrose, arginine, Tween 80, trehalose, phenylalanine, and malic acid was found to be positively correlated with soil organic matter, whereas microbial response to fumaric acid, citric acid, alanine, and glucose was negatively correlated with soil organic matter (Fig 1a). In addition, microbial response to arginine, phenylalanine, trehalose, Tween 80, malic acid, sucrose, and oxalic acid was negatively correlated with potato tuber sucrose content. In bulk soil, microbial response to arginine, phenylalanine, trehalose, Tween 80, malic acid, and sucrose was positively correlated with soil moisture, whereas response to fumaric acid, citric acid, and alanine was negatively correlated with soil moisture (Fig. 1b).
Laboratory experiment: potato litter decomposition
The C:N ratio of the potato shoot litter used in the decomposition experiment neither was significantly different between cultivars (F = 3.30, p = 0.07) nor was it significantly influenced by which field site (BUI or VMD) the plants had grown (F = 2.98, p = 0.11) (Fig. 2). Decomposition of this material in soil-litter incubations was significantly influenced by location in terms of which field site the soil and litter had been derived from (BUI or VMD); however, no effect of cultivar was found: whether the soil and litter originated from plots with Karnico, Modena, or Aventra did not significantly affect decomposition variables, at least in home situations (Table 3). In general, decomposition rates were higher in soil derived from location VMD than from BUI (Fig. 3). At location VMD, the C mineralization rate of Aventra litter in Aventra soil was lower as compared to Karnico litter in Karnico soil and Modena litter in Modena soil for the first 23 days of incubation (Fig. 3a). At location BUI, the C mineralization rate of Aventra litter in Aventra soil as compared to Karnico litter in Karnico soil and Modena litter in Modena soil was lower at day 65 of incubation. After 121 days of incubation, mineral N was higher in all soil-litter incubations, compared to soil-only incubations (Fig. 4a). However, microbial biomass N was only found to be higher in soil-litter incubations (compared to soil-only incubations) if the soil was derived from location VMD (Fig. 4b).
When comparing all treatments (both home situations and away situations), we detected a home-field advantage effect of Modena (if soil and litter were derived from VMD) and Karnico (soil and litter from BUI) on net N mineralization (Table 4). Furthermore, we found a significant home-field disadvantage effect of Aventra on N mineralization, regardless of whether the soil and litter were derived from location VMD and BUI. No home-field advantage (or disadvantage) effects were observed with regard to other decomposition variables (C mineralization and microbial biomass N).
Discussion
In this study, we assessed the effects of GM potato cultivar Modena on soil microbial activity and decomposition processes and compared these to effects induced by the parental line (Karnico) and a conventional cultivar (Aventra), both in the field and in a laboratory experiment. In the field experiment, effects of Modena on microbial catabolic diversity fell within the same range as effects induced by the non-GM potato varieties, both in rhizosphere and bulk soil. In the laboratory experiment, we detected cultivar-specific effects on N mineralization only, in terms of a home-field advantage effect of Modena (if soil and litter were derived from location VMD) and Karnico (soil and litter from BUI). Potato cultivar Aventra was found to induce a home-field disadvantage in net N mineralization, in soil-litter incubations from both VMD and BUI.
The field experiment
As said, microbial catabolic diversity did not differ significantly between field crops of the potato cultivars investigated, in rhizosphere nor bulk soil. However, multivariate analyses (RDA) indicated that a significant proportion of the variation in microbial catabolic diversity could be related to soil characteristics (soil moisture, organic matter) and plant traits (in particular, tuber sucrose content). Soil organic matter, a source of nutrients and energy, differed significantly between the two field locations (Table 1), and this had an overriding influence on the soil microbial activity, as has been observed in other studies (e.g., Fierer et al. 2009). In addition to organic matter, environmental factors such as temperature and water conditions also affect microbial activity. Orchard and Cook (1983) observed that soil water potential showed a positive log-linear relationship with soil microbial activity, as long as the latter was not limited by substrate availability, which is correlated to organic matter content. Such strong links between soil moisture and organic matter are well known (e.g., Gupta and Larson 1979; Vereecken et al. 1989) and were also observed in our study. Hence, the observed difference between the driving factors of microbial catabolic diversity in the rhizosphere (organic matter) versus bulk soil (soil moisture) is only relative.
Although we found notable differences between the microbial catabolic activity patterns of the two (BUI and VMD) locations, we found these activity patterns to be highly similar between the rhizosphere and bulk soil within the same location. At location VMD (loamy peat: high organic matter and moisture content), microorganisms in both rhizosphere and bulk soil were strongly activated on the same set of substrates (sucrose, arginine, Tween 80, trehalose, phenylalanine, and, to a lesser extent, malic acid). The soil microbial community at location BUI (loamy sand: low organic matter and moisture content) responded to a different subset of substrates (alanine, citric acid, and, to a lesser extent, fumaric acid and glucose), again without differences between rhizosphere and bulk soil. Our results show that the MicroRespTM methodology was effective in differentiating distinct microbial rhizosphere communities, as found by Knox et al. (2014) and Dieng et al. (2014). The absence of differences between rhizosphere and bulk soil suggests that either the microbial communities were not significantly affected by the potato roots or that the methodology was not sensitive enough to detect relatively small effects on the microbial activity.
The only potato cultivar-related effect detected was the effect of tuber sucrose content, which explained 2.4 % of the variation in rhizosphere microbial respiration. It seems unlikely that tuber sucrose directly affected the rhizosphere microbial community through sucrose as a leachate, because experimental addition of sucrose to rhizosphere soil from potato tubers with high tuber sucrose content resulted in reduced, rather than increased, microbial activity (MicroRespTM; Fig. 1a). However, the MicroRespTM tests did show a positive (albeit small) effect of glucose, which is the breakdown product of sucrose, on the activity of the rhizosphere microbial community of high tuber sucrose potato tubers. These results suggest that the potato plants did have some effect on the activity of rhizosphere microorganisms, but any effects in bulk soil remained below the detection limit of the MicroRespTM method.
The laboratory experiment
In the laboratory experiment, potato cultivar effects were observed for N mineralization, but not for C mineralization or microbial biomass N. In soil-only incubations, and in soil-litter incubations in which litter and soil had been derived from the same cultivar plots (home situations), cultivar had no detectable effect on decomposition variables. However, similar to our field observations, decomposition processes were significantly different between field site locations, with generally higher decomposition rates in incubations with soil derived from location VMD (high in organic matter) than from BUI (low organic matter). When comparing decomposition processes in home situations (soil and litter from the same cultivar plot) and away situations (soil and litter from different cultivar plots), some cultivar effects were detected, in terms of a home-field advantage effect of Modena (if soil and litter were derived from location VMD) and Karnico (soil and litter from BUI) on net N mineralization. Furthermore, Aventra was found to induce a home-field disadvantage in net N mineralization, in soil-litter incubations from both VMD and BUI. A home-field (dis)advantage generally results from changes in the decomposer community or differences in litter quality (Ayres et al. 2009). However, our MicroRespTM measurements did not indicate any significant differences between cultivars in terms of the microbial catabolic diversity in the bulk soil used for the incubation experiment nor did we find statistically significant differences in terms of litter C:N ratio (Fig. 2). Possibly, the observed home-field (dis)advantages were related to interactions between litter N and decomposer communities (Perez et al. 2013). The generally weak home-field effects found in our soil-litter incubations could be explained by the short duration of the field experiment from which the soil and litter were derived: in other studies measuring home-field effects, the plant-soil system had been in place for many more growing seasons than in our study (e.g., forests in Ayres et al. 2009).
Conclusions
We showed that soil microorganisms and related decomposition processes were not affected to a detectable degree by GM potato cultivar Modena, when compared with the parental line and a conventional cultivar. Our results also demonstrate the relevance of including variation in soil characteristics (e.g., effects of moisture conditions on respiration (Shi and Marschner 2015)), while quantifying effects induced by specific potato traits such as tuber sucrose content. Our results are in line with previous investigations on the effects of Modena on soil biota and soil processes (Hannula et al. 2012a, b; İnceoğlu et al. 2012; Vervoort 2013), which all indicate that this particular GM potato cultivar has either no measurable or no consistent effects on soil ecosystems, when compared to conventional cultivars. Based on the relevance of the soil biota investigated in these studies (bacteria, fungi, and nematodes; Hannula et al. 2012a, b; İnceoğlu et al. 2012; Vervoort 2013), which include effects on soil biodiversity, biomass distribution across trophic levels, and food web topologies, we conclude that Modena is unlikely to significantly affect soil ecosystems in terms of specific effects due to its genetic modification. However, it must be underlined that the results of these studies, including our experiments, concern the effects of a single plant trait, i.e., the knockdown of a specific gene in the carbohydrate metabolism of potato, and cannot be translated to other genetic modifications or other GM crops.
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Acknowledgments
We thank Maaike Bruinsma and Andreas Plischke for their collaboration in the field experiment and Jet Vervoort and Renée van der Salm for their help with the experimental work. Peter Bruinenberg and Paul Heeres from AVEBE/Karna and Thorsten Storck, Andreas Heise, and Christine Wandelt from BASF Plant Science Company GmbH are acknowledged for allowing us to take soil and plant samples from their experimental fields and to use their potato trait data. Christien Ettema from Shades of Green—De Groene Vertaler is acknowledged for improving the English. We are grateful to the two anonymous reviewers for their time and valuable suggestions for improvement on an earlier draft of this article. This research was financially supported by ERGO grants 838.06.061, 838.06.063, and 838.06.064 of the Netherlands Organization for Scientific Research (NWO, The Hague).
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Brolsma, K.M., Vonk, J.A., Hoffland, E. et al. Effects of GM potato Modena on soil microbial activity and litter decomposition fall within the range of effects found for two conventional cultivars. Biol Fertil Soils 51, 913–922 (2015). https://doi.org/10.1007/s00374-015-1031-2
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DOI: https://doi.org/10.1007/s00374-015-1031-2