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Volume 58, Issue 1

gen. nov., sp. nov., a butyrate-oxidizing, sulfate-reducing bacterium isolated from an anaerobic bioreactor Free

Abstract

A novel sulfate-reducing bacterium, strain HB1, was isolated from an upflow anaerobic sludge blanket (UASB) reactor treating paper-mill wastewater operated at 37 °C. Cells of strain HB1 were oval to rod-shaped, 1–1.3 μm wide and 2.6–3.5 μm long and Gram-negative. The optimum temperature for growth was 28–30 °C. In the presence of sulfate, the isolate was able to grow on H/acetate, formate, ethanol, propionate, fumarate, succinate, butyrate, crotonate, catechol, benzoate, 4-hydroxybenzoate, palmitate and stearate. The isolate only grew on H when acetate was added as a carbon source; when grown on formate, acetate was not required. Growth was also possible on pyruvate and crotonate without an electron acceptor. The isolate showed very poor growth on acetate. Thiosulfate and sulfate were used as electron acceptors. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain HB1 represents a novel lineage within the ; sequence similarities between strain HB1 and members of other related genera were less than 91 %. Strain HB1 was also distinguished from members of related genera based on differences in several phenotypic characteristics. It is a member of the family . The major cellular fatty acids of strain HB1 were C, iso-C, anteiso-C and C. -Hydroxy fatty acids were also present in the range of C to C, of which C was the most abundant. The G+C content of the DNA was 55.1 mol%. Based on physiological, biochemical and chemotaxonomic traits together with results of comparative 16S rRNA gene sequence analysis, strain HB1 is considered to represent a novel species in a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is HB1 (=DSM 18734 =JCM 14470).

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Keyword(s): UASB, upflow anaerobic sludge blanket
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2008-01-01
2024-04-19
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Desulfatirhabdium butyrativorans gen. nov., sp. nov., a butyrate-oxidizing, sulfate-reducing bacterium isolated from an anaerobic bioreactor
Int J Syst Evol Microbiol 58, 110 (2008); https://doi.org/10.1099/ijs.0.65396-0
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