Abstract
In densely populated areas, roadside verges often provide the last semi-natural habitats available. Their ecological value is often stressed by bio survey results. Yet insect summer surveys potentially misjudge the value of a biotope (roadside or otherwise) since the occurrences of species may only be seasonal, or even transient. To effectively ascertain the importance of a site for insects, species must be shown to complete their life cycle there or at least be shown to successfully accomplish critical life stages, such as reproduction or overwintering. To confirm overwintering of arthropods in roadsides, sods were cut in late winter in a verge where several years of summer survey data were available. The sods were placed in transparent semi-permeable cages and kept alive during subsequent spring, summer and autumn. All emerging arthropods were caught inside the cages using pitfall and funnel traps and identified to order or family level. Most of the terrestrial arthropod phyla and orders occurring in NW-Europe appeared to be represented. Several groups were further identified to the species level: Carabidae (Coleoptera), Curculionidae (Coleoptera), Araneae, Orthoptera, Apidae (Hymenoptera), Syrphidae (Diptera) and Dephacidae/Cercopidae (Hemiptera). Particularly for the Carabidae, Araneae and Curculionidae, many species recorded in summer were also found to overwinter; species overlap amounted to approximately 67%. Rarefaction of the summer sample or excluding possible summer vagrants, raised this overlap to as much as 88% for the Carabidae. Many of the species successfully overwintering in the roadside verge were generalists, but less common, more stenotopic species were present as well. Not only species hibernating as adults were involved, but also species overwintering in immature stages, indicating reproduction also takes place in the roadside verge. Apparently the roadside occurrence of many arthropod species, including stenotopic and declining ones, is not merely seasonal or incidental, and roadside verges do not necessarily act as a sink only. The ecological importance often attributed to roadside verges should clearly be taken seriously.
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Introduction
In many areas intensifying agriculture and ongoing urbanisation has resulted in considerable habitat loss for wildlife (Stoate et al. 2001; Geiger et al. 2010). In such landscapes roadside verges provide remaining (or sometimes even the last) semi-natural habitat for various organisms, especially arthropod and plant species (Way 1977; Dowdeswell 1987; Munguira and Thomas 1992; Sýkora et al. 1993; Vermeulen 1993; Eversham and Telfer 1994; Samways et al. 1997; Ries et al. 2001; Le Viol et al. 2008; Noordijk et al. 2009). Roadside verges also connect habitats and may thus function as corridors or stepping-stones in colonisation and meta-population processes (DeMers 1993; Vermeulen 1994; Noordijk 2009). These actual and potential values of roadside verges are widely recognised and in several countries policies and management schemes have been developed to conserve or enhance this ecological function (Anonymus 1984; Zwaenepoel 1998; RWS 2004; Keizer et al. 2006).
Yet, it is difficult to actually demonstrate the arthropod conservation value of individual roadside verges. Bio-surveys and arthropod assessments are usually carried out in spring or summer and focus almost exclusively on adult specimens (e.g. Ries et al. 2001; Koivula et al. 2005; Le Viol et al. 2008; Schaffers et al. 2008; Noordijk et al. 2009; Kotze et al. 2011). The results of such surveys do not unequivocally reveal the site’s contribution to the survival of the encountered species. Many arthropods are quite mobile and their ecology is poorly known (Dennis et al. 2007; Samways et al. 2010). The roadside occurrence of many arthropods, stenotopic species in particular, might just be seasonal or transient, with actual survival depending on the surrounding landscape (Thomas et al. 2001). Seasonal occurrence of arthropods has previously been described for farmland, where the long-term survival of most arthropods depends on field margins rather than on the fields themselves (Sotherton 1984, 1985). Vagrancy is a known problem in for instance butterfly recording schemes, leading to misjudgements of species statuses (Dennis 2001). In some situations it is even conceivable that roadside verges act as a habitat sink (Pulliam 1988; Vermeulen 1994; Eversham and Telfer 1994; Ries et al. 2001; Koivula 2005; Desender et al. 2010). Especially when roadside verges are attributed conservation objectives, it is important to gain a better understanding of their actual importance for arthropods.
To ascertain that roadside verges are truly important—i.e. to show that arthropods actually complete their life cycle in those habitats—is not easy. A multitude of species with a wide variety of life-strategies is involved. However, in temperate climates the life cycle of most arthropods shows a distinct annual pattern, generally involving a winter diapause (Tauber and Tauber 1976; Thiele 1977; Leather et al. 1993; Speight et al. 2008). During this hibernation period, eggs and hibernating individuals cannot anticipate on changing circumstances. It is therefore of crucial importance for a species to find suitable locations where this life history stage (hence called overwintering) can be successfully completed. This may warrant the generalisation that if a species overwinters successfully in a specific roadside verge, this verge may be considered to provide a substantial contribution to its local survival. Comparing the overwintering species composition to the results of a common summer survey will lead to a better understanding of the actual conservation value of a site. If similar species compositions are found, then both survey methods apparently are suitable for arthropod conservation value assessment.
The aims of the present study were to ascertain arthropod overwintering in roadside verges and to assess the relevance of common spring/summer bio-surveys. The following three main questions will be addressed in this study:
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(1)
Do arthropod species use roadside verges for overwintering and can they successfully complete their life cycle in the year following?
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(2)
Do also rare species or those adapted to a narrow range of environmental conditions (stenotopic species) utilize roadside verges?
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(3)
To what extent is the species composition of overwintering arthropods related to the species composition in a common spring/summer bio-survey?
Methods
Study area
The study area concerned the roadside verge of a main road (N-225) near Heelsum, the Netherlands (51°58′31.7″N-05°45′05″E). The vegetation consisted of a grass encroached Thero-Airion (Schaminée et al. 1996). This is a late stage pioneer community of dry, acid to neutral, base- and nutrient-poor sandy soils. Dominant and characteristic plant species were the grasses: Agrostis capillaris L. and Festuca filiformis Pourr., the herbs: Rumex acetosella L., Hypochaeris radicata L., Ornithopus perpusillus L., Jasione montana L., Teesdalia nudicaulis (L.) R.Br., and the mosses: Hypnum cupressiforme Hedw. and Polytrichum juniperinum Hedw. In the years before study, the verge had been mown every 2 or 3 years in autumn and hay was removed. The verge measured 30 m in width and the total area of the Thero-Airion community was about 750 m2. An extensively grazed pasture bordered the verge, but not the studied community.
Assessment of overwintering arthropods
In February 2001, 40 sods were cut in the roadside verge from an area of approx. 200–250 m2 within the Thero-Airion vegetation. Individual sods measured 0.25 × 0.50 m with a depth of 0.20 m; the total surface of all collected sods therefore amounted to 5 m2.
The sods were placed in five 1 by 1 m cages, located on a ploughed field at Wageningen University. These cages (Fig. 1) in fact concern modified emergence traps, which are commonly used to assess soil- and litter-overwintering arthropods (Southwood and Henderson 2000). The soil below the cages was sandy like the sods themselves, though richer in nutrients and more humid. The vegetation in the cages was kept alive until October to give emerging immature arthropods the opportunity to reach adulthood. This way the problem of identifying juveniles, which is difficult if possible at all, was avoided. The vegetation in the cages was watered if necessary, but although the cage top was made of acrylate, the wire gauze sides and bottom admitted enough rain and moisture to make watering necessary only occasionally. According to measurements performed in November, light intensity in the cages was approximately 60% of full daylight.
Adult arthropods were collected in the cages from early March until the middle of October. Per cage we used one pitfall trap to catch ground dwelling arthropods and one funnel fall in the top of each cage to collect flying arthropods. The pitfall traps were opened 2–3 days per week and checked once or twice a day. With this intensive removal scheme we intended to keep predation levels within the traps low. Adult arthropods were collected for identification, but trapped juveniles were released again. Despite regular checking juveniles were sometimes killed or mutilated by predators. The funnel traps were installed in the middle of April and filled with a 4%-formaldehyde solution for preservation. We aimed to catch full-grown, identifiable adults in these traps (assuming individuals must have been flying to enter these traps), but juveniles were nevertheless caught in the funnel traps occasionally and these individuals could not be released again. The following groups were identified to species level: Carabidae (Coleoptera), Curculionidae (Coleoptera), Orthoptera, Araneae, Syrphidae (Diptera), Apidae (Hymenoptera) and also the smaller groups Delphacidae and Cercopidae (both Hemiptera). Most other arthropods were identified to order or family level. Juveniles of the groups studied in detail could seldom be identified at species level and could only be included in total counts at higher levels.
Of the Araneae only epigeic, non-webbing species were considered. Webbing species were actively removed from the cages for two reasons: first of all they are very efficient predators and as such would heavily interfere with our trapping effort and secondly this group was also not sampled during the summer season bio-survey (see below) that was used to compare the winter data against.
For Carabidea we used two separate classifications for deciding whether or not a species should be considered stenotopic (for dry or wet habitats): an eurytopy measure of 5 or less in Turin (2000) or classification as stenotopic by Lindroth (1949).
The study did not include a null treatment to identify possible cage-invading species, even though the 1 mm wire gauze used for the cages most likely was not impenetrable for some very small, adult or juvenile, arthropods. However, the arthropod species composition of arable fields (on which the cages were placed) is known to be species poor and of quite a different nature than that of nutrient poor grasslands from which the sods originated (Sotherton 1984, 1985; Turin 2000), allowing most possible invaders to be recognized as such. With this in mind and with a fixed number of cages available, reducing the risk of revealing only a subset of the overwintering species (by using all cages) was considered more important than reducing the risk of inadvertently including potentially cage-invading ‘arable’ species (by assigning null treatments to one or more cages).
Assessment of arthropods in summer
The investigated roadside verge was part of a study concerning the relation between plant communities and invertebrate communities (Schaffers et al. 2008). For this study several arthropod taxa (the ones identified to species level mentioned before) were sampled during the growing seasons of 1998, 1999 and 2000, at the exact same roadside location and plant community where the sods were collected to assess overwintering. Carabidae and Curculionidae were collected from June to October 1998 and March to October 1999 using 5 pitfall traps (d = 9 cm, 5 m interspaced, filled with a 4% formol solution, and sheltered under a lid to keep out rain) which were pooled. Pitfall data on Curculionidae was completed with data from sweepnet catches (see below). For Araneae, only the 1998 pitfall catches were used and only epigeic, non-webbing species were considered. Apidae were collected using an insect net and using white cups. For the latter so-called ‘yellow pan method’, three cups (d = 9 cm) filled with a NaCl-solution were placed in the vegetation in May and August 2000, during 1 week of fair weather each time. Syrphidae were collected together with Apidae while the latter were the prime target. Orthoptera records were obtained by sound and sight observations, performed on various occasions with fair weather during 1998, 1999 and 2000. Cercopidae and Delphacidae (and additional Curculionidae, see above) were collected in 1998 and 1999 using a sweep net on four occasions each year. The combined result of these different sampling techniques were considered to reflect the arthropod composition of the site in the growing season.
Data analysis and statistics
For arthropod groups where both winter and summer catches were available (the groups identified to species level), overwintering species composition was compared to the results of the summer survey. As a measure of similarity we used the percentage species overlap, calculated as the number of species shared, relative to the number of species in summer. In addition, also the Jaccard index and the Sørensen similarity coefficient can be calculated, defined as the number of shared species relative to the total or the average number of species, respectively. In the presence-absence approach applied here the Sørensen similarity equals the one-complement of the Bray-Curtis dissimilarity (Legendre and Legendre 1998). We also calculated Spearman rank correlations between summer and winter abundances.
For a meaningful comparison between winter and summer catches, however, several aspects deserve attention. First, we need to address differences in sampling intensity. Summer sampling collects actively moving individuals and thus tends to effectively cover a wide area, whereas the winter sample was relatively small and collected over a fixed area. The summer survey thus resulted in the capture of much more individuals than obtained from the 5 m2 of sods cut in winter. We used rarefaction (Hurlbert 1971) to compensate for this. We rarefied the summer sample to the number of individuals caught in winter. The expected richness of the rarefied summer sample was calculated using the equations given by Hurlbert (1971). To assess the species overlap after rarefaction, we first ranked the summer catches according to species abundance and only retained the k species with the highest number of individuals, with k being the rarefied richness. If k was a fractional number, or if the abundance of the kth species was shared by others, interpolation was used to calculate the overlap.
As an alternative for rarefaction, we deleted possible summer vagrants from the summer data before calculating the overlap. Suspected vagrants were provisionally defined here as those species with less than four individuals in the entire summer sample, irrespective of the winter data.
Apart from these adjustments to the summer survey, we also need to ascertain that the winter survey was extensive enough to have recorded most of the overwintering species. To ascertain the comprehensiveness of the winter sample we studied the shape of rarefaction curves. We did not rarefy individuals but used rarefaction based on samples (the overwintering cages), thus allowing for sample heterogeneity. We determined the number of trapped species for all possible combinations of 0–5 cages (n = 32). The so obtained rarefaction curves essentially correspond to species-area curves covering 0–5 m2 of soil sods.
Under the assumption that species numbers should level off with growing sample size, we tested the comprehensiveness of the sample by fitting both a linear and a logarithmic model to the rarefaction curve. If the fit of the exponential model turns out to little better than that of the linear model, sample size has likely not been sufficient since no obvious levelling-off can be detected in the rarefaction curve. If on the other hand the logarithmic model clearly fits the observations better, the species increase is apparently levelling-off within the 5 m2 of sods sampled and sample size may be concluded to have been more or less sufficient (depending on the amount of levelling-off).
Both models tested were required to pass through the origin (0 m2: 0 spp.). The linear model applied was therefore of the form y = β·x; the logarithmic model used was y = α·ln(βx + 1). To fairly judge the difference between the two models we divided the relative increase in R2 by the relative change in degrees of freedom, leading to an F statistic. F values greater than 1 indicate that model fit has changed more than would be expected by the change in degrees of freedom. We cannot derive an exact significance value for the difference between the models using this F statistic because the individual data points are not statistically independent in our case. But we can use the critical values of the distribution to obtain a general impression of the difference in appropriateness of the two models.
Results
Overwintering arthropods
Adult representatives of most terrestrial, non-parasitic arthropod phyla and orders occurring in NW-Europe were collected in the cages (Table 1). The number of individuals and species per taxon differed considerably and some taxa were represented poorly, like the species-rich Lepidoptera. At family level absences started to occur. Most of the larger families within the Coleoptera and Araneae were still present but within the Hymenoptera Aculeata, for instance, larger families like Vespidae, Pompilidae and Chrysididae were absent. Some species and higher taxa were collected in rather large numbers, notably Staphylinidae and Diptera-Nematocera. Observations revealed that some species were not trapped very efficiently. Homoptera were frequently seen in the cages but hardly occurred in the traps and in early summer hundreds of dead Diptera-Nematocera were lying on the edges of the side panes where they could not be collected and were subsequently eaten by other taxa.
The species collected not only include species hibernating as adults like many Carabidae and Staphylinidae, but also species overwintering in immature stages. This can be deducted from the biology of the collected species, but is also illustrated by the continued appearance of additional taxa until the end of summer (Fig. 2).
Most collected species are generalists but less common, more stenotopic species from various orders were present as well. Examples of species characteristic of nutrient poor, sandy grasslands and heathlands are the grasshopper Stenobothrus stigmaticus (Rambur), the digger wasp Didineis lunicornis (Fabricius), the robber fly Machimus cingulatus (Fabricius), the wingless ground beetle Poecilus lepidus (Leske) and the solitary bee Panurgus calcaratus (Scopoli).
Species composition
The cage results show that many of the Carabidae, Araneae and Curculionidae species recorded in the summer survey, also overwinter in the studied roadside verge (Table 2). For these three groups, the species overlap (relative to the summer catches) amounts to 60–67%. Rarefaction of the summer sample to correct for differences in sampling intensity, raised the overlap to 68–84% for these three groups. The alternative of excluding possible summer vagrants (less than four individuals in summer) even raises the overlap to 88% for Carabidae and 86% for Curculionidae. Yet, some of the Carabidae and other species that were only caught in scarce amounts during summer did emerge in the cages, sometimes even quite numerous compared to others. We also collected additional species from the cages, species that were not recorded during summer, especially Carabidae (Table 3) and some Araneae.
For Orthoptera the encountered species overlap was 50%, raising slightly after rarefaction of the summer sample. For Apidae, Syrphidae and Delphacidae/Cercopidae the overlap is rather low (20% or less). Rarefaction or removing possible vagrants increases the overlap, but for these groups the summer data is not very well suited for rarefaction or deleting possible vagrants due to their high mobility and/or the low numbers captured.
Of the seven declining Carabidae species caught during the summer surveys, three also emerged in the cages (Table 3). Of the remaining four, two had been represented by only one individual in the summer survey. Of the stenotopic species caught during summer approximately 50% were present in the overwintering cages, regardless of whether Turin’s (2000) or Lindroth’s (1949) classification of biotope specificity is used (Table 3). In this case also, most of the species ‘missing’ in the cages were rare recordings during summer (possible vagrants). Of the eight species only found in the cages, two are stenotopic according to Turin, and four according to Lindroth. Surprisingly, of the four ‘cage-only’ species classified as stenotopic by Lindroth, three prefer wet biotopes. However, two of these (very small Bembidion species with only one or two individuals each) might be accidental cage-invasions. The grasshopper Stenobothrus lineatus (Panzer) and the solitary bee Andrena ovatula (Kirby), both vulnerable species in the Netherlands (Odé et al. 1999; Peeters and Reemer 2003), were recorded in low densities during the summer survey but did not emerge in the cages.
In general, Carabidae species that were more abundant in the summer survey, were indeed more numerous in the winter sample and visa-versa (Spearman’s ρ = 0.442; n = 38; one-sided P = 0.003). For the Araneae such a positive relationship is also present, but significant only if the analysis is restricted to the overlapping species (ρ = 0.581; n = 11; P = 0.03). For the Curculionidae the number of species is quite low, but nevertheless an indication exists for a positive correlation (ρ = 0.458; n = 12; P = 0.07), partly because the species that was most common in summer also appeared to be the most common in the overwintering cages.
Comprehensiveness of winter assessment
For both the Carabidae (Fig. 3a) and Araneae (Fig. 3b) the logarithmic models fitted the rarefied winter data better than the linear model (F[1,30] = 159.5 for Carabidae and 40.55 for Araneae; with critical values for α = 0.05 at 4.17, for α = 0.01 at 7.56, and for α = 0.0001 at 20.1). This indicates a clear saturation of species numbers within the surface area of sods used (0–5 m2) although the levelling-off is less pronounced for the Araneae.
The Curculionidae (Fig. 3c) show relatively low fit values for both models due to the large amount of scatter which, in turn, is caused by the low number of species caught. Because of this there is little difference between the fit of the two models (F[1,30] = 2.98, with the critical value for α = 0.10 at 2.88), indicating that for this group there is no clear sign of species saturation yet.
Discussion
The roadside verge appears to be used as an overwintering site for a large number of arthropod groups and a high level of overlap was demonstrated between the overwintering species and the species encountered in a common bio-survey in summer. This holds particularly for the Carabidae, Araneae and Curculionidae (and to a lesser extent also the Orthoptera). Although winter sampling was limited to 5 m2 of soil sods, a large proportion of the overwintering species has likely been identified, as clear levelling-off could be demonstrated in some of the rarefaction curves (Fig. 3). Particularly for the Carabidae and Araneae these curves suggest that species numbers would probably not have been much higher with larger sample sizes, which is in accordance with the clear overlap in species composition between overwintering species and the species observed during summer surveys for these groups (Table 3). For the Curculionidae, species saturation could not be clearly demonstrated due to the low number of species, but also for this group confidence in the winter data is strengthened by the similarity in summer and winter composition, which was of comparable magnitude to that of the Carabidae.
Our results seem to validate summer sampling (the most common method to assess arthropod conservation value) as an effective bio-survey. However, this is only true because in our case overwintering was positively confirmed for a large proportion of the species caught in summer. Without such confirmation, sampling under optimum conditions and restricted to particular life history stages may still not provide us with a correct indication of the actual habitat value of a site (Dover and Settele 2009). An interesting observation on the efficiency of summer sampling can be made if we examine the species numbers after rarefication of the summer survey to the size of the winter sample (Table 2). For Carabidae, Araneae and Curculionidae the winter sampling of soil sods then appears to have been more effective than the summer survey (26 vs. 18, 15 vs. 9.9 and 8 vs. 6.3 species, respectively). It is only because large numbers of (active) individuals can be trapped with limited effort that summer surveys appear more effective for these groups.
For the species that also turned up in the winter survey (the overlap) the summer survey is indeed validated. Yet the reverse conclusion, that taxa must have been coincidental or seasonal vagrants if only present in the summer survey, is not necessarily true. For these species the situation is unclear since emerging individuals only reveal methodological success. Absences from the winter cages can have different explanations than the site being unsuitable for successful overwintering. Various methodological aspects may be responsible.
First of all, cage induced circumstances may have prohibited individuals to survive and reach adulthood (Southwood and Siddorn 1965). Secondly, trapping in the cage may not have been fully efficient and this may cause some species to have been missed. Thirdly, sample size may not have been sufficient and some species may not have been included in the sampled sods. This particularly holds for rare species and for species overwintering in clusters, which is not uncommon and occurs in many arthropod groups (Allee 1931; Thiele 1977; Leather et al. 1993; Andersen 2011). For such species, larger sample sizes may be needed to establish their presence. A final reason may be the deliberate selection of homogeneous vegetation for sampling of sods. Many species rather prefer sites with more variation for overwintering; e.g. at the boundary between vegetation types, in heterogeneous patches near disturbances, under accumulated litter, near the edge of a ditch, near tussocks or molehills, etc. (Dennis et al. 1997; Woodcock et al. 2007).
From cage observations and the ecology of the studied taxa, we can often deduce whether the absence of species is mainly due to actual unsuitability of the site or to methodological circumstances. Many overwintering Orthoptera may have been missed due to methodological problems. Of several tens of individuals in the cages in spring, only five reached adulthood. Juveniles (which could not yet be identified at the species level) often climbed to the cage roof and fell into the funnel traps (recorded as Orthoptera in Table 1, but not identified at species level in Table 2). For species occurring in low densities, like Stenobothrus lineatus, sample size (area of sods) may have been limiting as well. For Delphacidae/Cercopidae and other Homoptera the trapping methods in the cages were rather inefficient. Many individuals were observed in the cages but relatively few were actually collected.
The low correspondence between summer and winter catches for Syrphidae and Apidae can partly be ascribed to the large individual dispersal ranges of these flying flower-visitors, which may give them a high chance of detection in summer. For the Syrphidae for instance, mainly very common and mobile species were recorded in summer; these may well have been seasonal or accidental visitors. Rarefaction of the summer sample (with strong effects for both Syrphidae and Apidae) might have compensated for this, but still did not lead to satisfactory levels of species cross-over. Apparently, site unsuitability or other ecological aspects also play a role here. For the Syrphidae only few of the species observed in summer actually hibernate as adults (e.g. Eristalis tenax (Linnaeus) and Episyrphus balteatus (De Geer)). The others hibernate in immature stages near the oviposition site. The absence of most of these is understandable, as their larvae live as (semi-)aquatic filter-feeders, as predators of aphids in trees, or in mouldered wood or dung (Rotheray 1993). For many Apidae species efficient winter sampling is complicated as nesting and therefore overwintering is highly clustered (Michener et al. 1958; Batra 1978; Rosenheim 1990; Westrich 1990). Another complication for some species is their use of so called partial habitats, which is particularly well described for bees (Westrich 1996). For such species, reproduction requirements (e.g., nest site, nesting material and food for brood provisioning), can only be met by spatially separated areas lying within limited distances of each other.
For some species groups, particularly Carabidae, the overwintering species composition appeared to be more than just a subset of the summer species composition, as overwintering species were found that were not recorded in the summer survey. A remarkably large proportion of the additional Carabidae species was characteristic of wet conditions (Table 3). As the studied site lies near the river Rhine, not far from the highest inundation levels, some of these additional ‘wet’ species might be species evading flooding or overwintering away from water bodies. The single specimen of Pterostichus gracilis (Dejean), the only ‘wet’ species recorded in the summer survey, was collected in April and may well fall in this category (possibly concerning an individual that had just left its overwintering site). The presence of such species could therefore indicate seasonal occurrence but, unlike the farmland situation described by Sotherton (1984, 1985), this would concern a winter instead of a summer influx. Migration to specific and distant winter biotopes is mentioned by Van Huizen (1977) for Amara plebeja (Gyllenhal), which is said to retreat to woodlands in autumn and hibernate there while recolonising grasslands during the growing season. But as this species also emerged from the grassland sods in the cages during our study this migratory behaviour is probably not strongly developed.
The results of this study show that overwintering in roadside verges is not confined to species that overwinter as adults. The emergence of species that overwinter as eggs (e.g. Orthoptera) or in larval stages at the oviposition site (e.g., Apidae and Crabonidae) implies that verges can also be used for actual reproduction.
Distinguishing between the actual source or sink character of our site is not as clear-cut as it may seem in theory. For instance Pulliam (1988) argued that it might be common for species to have a large fraction of their individuals occurring in “sink” habitats (maintained by surplus individuals from, possibly even small, source habitats) and that this may be ecologically stable. Watkinson and Sutherland (1995) showed that sometimes populations may appear as sinks (mortality exceeding reproduction) despite these populations being entirely viable in the absence of immigration. Such “pseudo-sinks” occur when immigration into the population reduces reproduction or increases mortality due to density dependent effects, resulting in a population that appears to be maintained by this immigration.
Such arguments imply that even if detailed quantitative data on reproduction, mortality and immigration would have been available, we would not be able to decide from this data on the actual character of our site in terms of source, sink or pseudo-sink, neither would this reveal the ecological relevance of the site in a meta-population context. However, if a sink is simply defined as an area where factors are insufficient for a species to carry out its life history (Pulliam 1988), we can conclude that the study site clearly did not act only as a sink, as it apparently provides resources for the persistence of a range of arthropod species and functions as a year-round habitat.
It could be argued that the studied roadside concerns a relatively wide verge, receiving a more conservation-friendly form of management than most other verges. However, roadsides are quite often much wider than the first one or two meters many people usually visualise (Schaffers 2000). Particularly along highways and motorways the habitat managed by road authorities is often several meters wide (even up to twenty or more) and generally receives an extensive form of management (Schaffers 2000). Nevertheless, more intensively managed roadside verges should be studied as well to see if also they have a similar capacity to support insect overwintering.
The results of this study demonstrate that roadside verges are used as overwintering sites by many arthropod taxa and that verges can also be used for actual reproduction. Apparently, the roadside occurrence of various arthropod species, including stenotopic and declining ones, is not merely seasonal or incidental, and roadside verges do not act as a sink only. Many species are capable of completing their entire life cycle in the roadside habitat, and in some cases the roadside verge may even act as a refuge (Eversham and Telfer 1994). These results imply that the ecological importance often attributed to roadside verges should clearly be taken seriously, at least as far as the habitat function for arthropods is concerned. Roadside verges truly are capable of performing conservation objectives.
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Acknowledgments
We are much indebted to Jinze Noordijk (European Invertebrate Survey—Netherlands) and two anonymous referees for helpful comments on the manuscript. This study was financially supported by the DVS Centre for Transport and Navigation of the Dutch Ministry of Infrastructure and the Environment.
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Open Access This is an open access article distributed under the terms of the Creative Commons Attribution Noncommercial License (https://creativecommons.org/licenses/by-nc/2.0), which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
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Schaffers, A.P., Raemakers, I.P. & Sýkora, K.V. Successful overwintering of arthropods in roadside verges. J Insect Conserv 16, 511–522 (2012). https://doi.org/10.1007/s10841-011-9437-0
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DOI: https://doi.org/10.1007/s10841-011-9437-0