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Series GSE106800 Query DataSets for GSE106800
Status Public on Aug 10, 2018
Title Circadian misalignment induces fatty acid metabolism gene profiles and induces insulin resistance in human skeletal muscle.
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Circadian misalignment, such as in shift work, has been associated with obesity and type 2 diabetes, however, direct effects of circadian misalignment on skeletal muscle insulin sensitivity and muscle molecular circadian clock have never been investigated in humans. Here we investigated insulin sensitivity and muscle metabolism in fourteen healthy young lean men (age 22.4 ± 2.8 years; BMI 22.3 ± 2.1 kg/m2 [mean ± SD]) after a 3-day control protocol and a 3.5-day misalignment protocol induced by a 12-h rapid shift of the behavioral cycle. We show that circadian misalignment results in a significant decrease in peripheral insulin sensitivity due to a reduced skeletal muscle non-oxidative glucose disposal (Rate of disappearance: 23.7 ± 2.4 vs. 18.4 ± 1.4 mg/kg/min; control vs. misalignment; p=0.024). Fasting glucose and FFA levels as well as sleeping metabolic rate were higher during circadian misalignment. Molecular analysis of skeletal muscle biopsies revealed that the molecular circadian clock was not aligned to the new behavourial rhythm, and microarray analysis revealed the human PPAR pathway as a key player in the disturbed energy metabolism upon circadian misallignement. Our findings may provide a mechanism underlying the increased risk of type 2 diabetes among shift workers.
 
Overall design Microarray analysis was performed on skeletal muscle biopsies (m. vastus lateralis) from healthy lean young men in a circadian misalignment study.
 
Contributor(s) Wefers J, van Moorsel D, Hansen J, Hooiveld GJ, Kersten S, Schrauwen P
Citation(s) 29987027
Submission date Nov 13, 2017
Last update date Aug 12, 2018
Contact name Guido Hooiveld
E-mail(s) guido.hooiveld@wur.nl
Organization name Wageningen University
Department Div. Human Nutrition & Health
Lab Nutrition, Metabolism & Genomics Group
Street address HELIX, Stippeneng 4
City Wageningen
ZIP/Postal code NL-6708WE
Country Netherlands
 
Platforms (1)
GPL17692 [HuGene-2_1-st] Affymetrix Human Gene 2.1 ST Array [transcript (gene) version]
Samples (47)
GSM2850460 skeletal muscle, circadian aligned intervention, evening (baseline) sample, subject RAS1
GSM2850461 skeletal muscle, circadian aligned intervention, morning sample, subject RAS1
GSM2850462 skeletal muscle, circadian misaligned intervention, evening (baseline) sample, subject RAS1
Relations
BioProject PRJNA418092

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Supplementary file Size Download File type/resource
GSE106800_RAW.tar 225.7 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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