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Series GSE8344 Query DataSets for GSE8344
Status Public on Jul 04, 2007
Title Effect PPARb/d knockout in white adipose tissue
Organism Mus musculus
Experiment type Expression profiling by array
Summary Analysis of white adipose tissue of PPARb/d knockout mice. Data may point towards putative target genes of PPARb/d and thus the function of PPARb/d in white adipose tissue. Datasets were used to identify glycogen synthase 2 as novel PPAR target.
Keywords: gene expression array-based, count
 
Overall design Three month old male PPARb/d knock-out mice were on a mixed background (Sv129/C57BL/6). Wild-type littermates served as control animals. Animals received standard chow. Epididymal white adipose tissue (WAT) was removed and total RNA was isolated and pooled afterwards (five animals per group). Pooled RNA was hybridized to Affymetrix mouse genome 430 2.0 arrays arrays. Five microgram total RNA was labeled according to the ENZO-protocol, fragmented and hybridized according to Affymetrix protocols.
 
Contributor(s) Mandard S, Wahli W, Muller M, Desvergne B, Kersten S
Citation(s) 17437057
Submission date Jul 01, 2007
Last update date Jan 08, 2019
Contact name Guido Hooiveld
E-mail(s) guido.hooiveld@wur.nl
Organization name Wageningen University
Department Div. Human Nutrition & Health
Lab Nutrition, Metabolism & Genomics Group
Street address HELIX, Stippeneng 4
City Wageningen
ZIP/Postal code NL-6708WE
Country Netherlands
 
Platforms (1)
GPL339 [MOE430A] Affymetrix Mouse Expression 430A Array
Samples (2)
GSM206810 WAT_wildtype_control_pool
GSM206811 WAT_PPARb-knockout_control_pool
Relations
BioProject PRJNA101351

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE8344_RAW.tar 7.2 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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