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Effect of condensed tannins in hulls of faba beans (Vicia faba L.) on the activities of trypsin (EC 2.4.21.4) and chymotrypsin (EC 2.4.21.1) in digesta collected from the small intestine of pigs

Published online by Cambridge University Press:  17 March 2008

A. J. M. Jansman ,
H. Enting ,
M. W. A. Verstegen  and
J. Huisman
A. J. M. Jansman
Affiliation:
Agricultural University, Department of Animal Nutrition, Haagsteeg 4, 6708 PM Wageningen, The Netherlands TNO Nutrition and Food Research Institute, Department of Animal Nutrition and Physiology (ILOB), P.O. Box 15, 6700 A A Wageningen, The Netherlands
H. Enting
Affiliation:
Agricultural University, Department of Animal Nutrition, Haagsteeg 4, 6708 PM Wageningen, The Netherlands
M. W. A. Verstegen
Affiliation:
Agricultural University, Department of Animal Nutrition, Haagsteeg 4, 6708 PM Wageningen, The Netherlands
J. Huisman
Affiliation:
TNO Nutrition and Food Research Institute, Department of Animal Nutrition and Physiology (ILOB), P.O. Box 15, 6700 A A Wageningen, The Netherlands
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Abstract

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The effects of condensed tannins in hulls of faba beans (Vicia faba L.) on the activities of trypsin (EC3.4.21.4) and chymotrypsin (EC3.4.21.1) in digesta obtained from the small intestine of pigs were studied. Using four castrated male pigs (mean body weight 83 kg) fitted with both a simple T-cannuIa in the duodenum and a post-valvular T-cannula at the terminal ileum, two experimental diets were tested in a Latin square design. The low-tannin diet (LT) contained 200 g faba bean hulls (cv. Blandine)/kg with a low content of condensed tannins (< 0.1 % catechin equivalents). The high-tannin diet (HT) contained 200 g faba bean hulls (cv. Alfred)/kg with a content of condensed tannins of 3.5 % catechin equivalents. Spot samples of fresh duodenal digesta were taken daily at fifteen time points between 08.00 and 20.00 hours on four consecutive days. Ileal digesta were collected nearly quantitatively on the same days between 08.00 and 20.00 hours over periods of 2 h. Trypsin and chymotrypsin activities in duodenal digesta did not differ between treatments at any time point (P > 0.05). In ileal digesta of pigs given diet HT the mean activity of trypsin was reduced (P < 0.05). The activity of chymotrypsin in ileal digesta did not differ between treatments. Trypsin activity: chymotrypsin activity was somewhat lower in ileal digesta of pigs receiving the HT diet (P < 0.10). The apparent ileal digestibility of crude protein (N × 6.25) was lower for the HT than for the LT diet (0.614 v. 0.728; P < 0.05). Condensed tannins are probably responsible for the lower activity of trypsin in ileal digesta of pigs fed on high-tannin faba bean hulls. Various explanations for the absence of effects of condensed tannins on enzyme activity in duodenal digesta are discussed.

Keywords

Condensed tanninsTrypsinChymotrypsinPigs
Type
Research Article
Information
British Journal of Nutrition , Volume 71 , Issue 4 , April 1994 , pp. 627 - 641
Copyright
Copyright © The Nutrition Society 1994

References

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